Target peptides for colorectal cancer therapy and diagnostics

ABSTRACT

A set of target peptides are presented by HLA A*0201, B*0301, B*0702 and B*2705 on the surface of disease cells. They are envisioned to, among other things, stimulate an immune response to the proliferative disease, e.g., colorectal cancer, to function as immunotherapeutics in adoptive T cell therapy or as a vaccine, facilitate antibody recognition of tumor boundaries in surgical pathology samples, act as biomarkers for early detection and/or diagnosis of the disease, and/or act as targets in the generation antibody-like molecules which recognize the target-peptide/MHC complex.

CROSS REFERENCE TO RELATED APPLICATIONS

The presently disclosed subject matter claims the benefit of U.S. patent application Ser. No. 14/425,946, filed Mar. 4, 2015 (now pending); which is a U.S. National Stage Application of PCT International Patent Application Serial No. PCT/US2013/058255, filed Sep. 5, 2013; which itself claims the benefit of U.S. 10 Provisional Patent Application Ser. Nos. 61/697,274, filed Sep. 5, 2012; 61/712,807 filed Oct. 12, 2012; and 61/736,466, filed Dec. 12, 2012. The disclosure of each of these applications is incorporated herein by reference in its entirety.

GRANT STATEMENT

This invention was made with government support under Grant No. AI033993 awarded by the National Institutes of Health. The government has certain rights in the invention.

REFERENCE TO SEQUENCE LISTING

The Sequence Listing associated with the instant disclosure has been electronically submitted to the United States Patent and Trademark Office as a 106 kilobyte ASCII text file created on Jun. 15, 2020 and entitled “3062_4_2_PCT_US_CON_ST25.txt”. The Sequence Listing submitted via EFS-Web is hereby incorporated by reference in its entirety.

TECHNICAL FIELD

The presently disclosed subject matter is related to the area of diagnostics and therapeutics. In particular, it relates to immunotherapies and diagnostics in the context of proliferative diseases such as cancer.

BACKGROUND

The mammalian immune system has evolved a variety of mechanisms to protect the host from cancerous cells. An important component of this response is mediated by cells referred to as T cells. Cytotoxic T lymphocytes (CTL) are specialized T cells that primarily function by recognizing and killing cancerous cells or infected cells, but they can also function by secreting soluble molecules referred to as cytokines that can mediate a variety of effects on the immune system. T helper cells primarily function by recognizing antigen on specialized antigen presenting cells, and in turn secreting cytokines that activate B cells, T cells, and macrophages. A variety of evidence suggests that immunotherapy designed to stimulate a tumor-specific CTL response would be effective in controlling cancer. For example, it has been shown that human CTL recognize sarcomas (Slovin et al., 1986), renal cell carcinomas (Schendel et al., 1993), colorectal carcinomas (Jacob et al., 1997), ovarian carcinomas (Peoples et al., 1993), pancreatic carcinomas (Peiper et al., 1997), squamous tumors of the head and neck (Yasumura et al., 1993), and squamous carcinomas of the lung (Slingluff et al., 1994; Yoshino et al., 1994). The largest number of reports of human tumor-reactive CTLs, however, has concerned melanomas (Boon et al., 1994).

The ability of tumor-specific CTL to mediate tumor regression, in both human (Parmiani et al., 2002; Weber, 2002) and animal models, suggests that methods directed at increasing CTL activity would likely have a beneficial effect with respect to tumor treatment.

Colorectal cancer (CRC), commonly also known as colon cancer or bowel cancer, is a cancer from uncontrolled cell growth in the colon or rectum (parts of the large intestine), or in the appendix. Symptoms typically include rectal bleeding and anemia which are sometimes associated with weight loss and changes in bowel habits. Most colorectal cancer occurs due to lifestyle and increasing age with only a minority of cases associated with underlying genetic disorders. It typically starts in the lining of the bowel and if left untreated, can grow into the muscle layers underneath, and then through the bowel wall. Screening is effective at decreasing the chance of dying from colorectal cancer and is recommended starting at the age of 50 and continuing until a person is 75 years old. Localized bowel cancer is usually diagnosed through sigmoidoscopy or colonoscopy. Cancers that are confined within the wall of the colon are often curable with surgery while cancer that has spread widely around the body is usually not curable and management then focuses on extending the person's life via chemotherapy and improving quality of life. Colorectal cancer is the third most commonly diagnosed cancer in the world, but it is more common in developed countries. Around 60% of cases were diagnosed in the developed world. It is estimated that worldwide in 2008, 1.23 million new cases of colorectal cancer were clinically diagnosed, and that it killed 608,000 people.

In Europe, the five year survival for colorectal cancer is less than 60%. In the developed world, about a third of people who get the disease die from it. Survival is directly related to detection and the type of cancer involved, but overall is poor for symptomatic cancers, as they are typically quite advanced. Survival rates for early stage detection are about five times that of late stage cancers. For example, patients with a tumor that has not breached the muscularis mucosa (TNM stage Tis, N0, M0) have an average 5-year survival of 100%, while those with an invasive cancer, i.e. T1 (within the submucosal layer) or T2 (within the muscular layer) cancer have an average 5-year survival of approximately 90%. Those with a more invasive tumor, yet without node involvement (T3-4, N0, M0) have an average 5-year survival of approximately 70%. Patients with positive regional lymph nodes (any T, N1-3, M0) have an average 5-year survival of approximately 40%, while those with distant metastases (any T, any N, M1) have an average 5-year survival of approximately 5%.

According to the American Cancer Society statistics in 2006, over 20% of patients present with metastatic (Stage IV) colorectal cancer at the time of diagnosis, and up to 25% of this group will have isolated liver metastasis that is potentially resectable. Lesions which undergo curative resection have demonstrated 5-year survival outcomes now exceeding 50%. Nevertheless, additional therapeutics that are safer and more effective than current therapies are in high demand.

In order for CTL to kill or secrete cytokines in response to a cancer cell, the CTL must first recognize the cancer cell (Townsend & Bodmer, 1989). This process involves the interaction of the T cell receptor, located on the surface of the CTL, with what is generically referred to as an MHC-peptide complex which is located on the surface of the cancerous cell. Major histocompatibility complex (MHC)-encoded molecules have been subdivided into two types, and are referred to as class I and class II MHC-encoded molecules. In the human immune system, MHC molecules are referred to as human leukocyte antigens (HLA). Within the MHC complex, located on chromosome six, are three different loci that encode for class I MHC molecules. MHC molecules encoded at these loci are referred to as HLA-A, HLA-B, and HLA-C. The genes that can be encoded at each of these loci are extremely polymorphic, and thus, different individuals within the population express different class I MHC molecules on the surface of their cells. HLA-A1, HLA-A2, HLA-A3, HLA-B7, HLA-B14, HLA-B27, and HLA-B44 are examples of different class I MHC molecules that can be expressed from these loci.

The peptides which associate with the MHC molecules can either be derived from proteins made within the cell, in which case they typically associate with class I MHC molecules (Rock & Goldberg, 1999); or they can be derived from proteins which are acquired from outside of the cell, in which case they typically associate with class II MHC molecules (Watts, 1997). The peptides that evoke a cancer-specific CTL response most typically associate with class I MHC molecules. The peptides themselves are typically nine amino acids in length, but can vary from a minimum length of eight amino acids to a maximum of fourteen amino acids in length. Tumor antigens may also bind to class II MHC molecules on antigen presenting cells and provoke a T helper cell response. The peptides that bind to class II MHC molecules are generally twelve to nineteen amino acids in length, but can be as short as ten amino acids and as long as thirty amino acids.

The process by which intact proteins are degraded into peptides is referred to as antigen processing. Two major pathways of antigen processing occur within cells (Rock & Goldberg, 1999). One pathway, which is largely restricted to professional antigen presenting cells such as dendritic cells, macrophages, and B cells, degrades proteins that are typically phagocytosed or endocytosed into the cell. Peptides derived from this pathway can be presented on either class I or to class II MHC molecules. A second pathway of antigen processing is present in essentially all cells of the body. This second pathway primarily degrades proteins that are made within the cells, and the peptides derived from this pathway primarily bind to class I MHC molecules. Antigen processing by this latter pathway involves polypeptide synthesis and proteolysis in the cytoplasm, followed by transport of peptides to the plasma membrane for presentation. These peptides, initially being transported into the endoplasmic reticulum of the cell, become associated with newly synthesized class I MHC molecules and the resulting complexes are then transported to the cell surface. Peptides derived from membrane and secreted proteins have also been identified. In some cases these peptides correspond to the signal sequence of the proteins which is cleaved from the protein by the signal peptidase. In other cases, it is thought that some fraction of the membrane and secreted proteins are transported from the endoplasmic reticulum into the cytoplasm where processing subsequently occurs. Once bound to the class I MHC molecule, the peptides are recognized by antigen-specific receptors on CTL. Several methods have been developed to identify the peptides recognized by CTL, each method of which relies on the ability of a CTL to recognize and kill only those cells expressing the appropriate class I MHC molecule with the peptide bound to it. Mere expression of the class I MHC molecule is insufficient to trigger the CTL to kill the target cell if the antigenic peptide is not bound to the class I MHC molecule. Such peptides can be derived from a non-self source, such as a pathogen (for example, following the infection of a cell by a bacterium or a virus) or from a self-derived protein within a cell, such as a cancerous cell. The tumor antigens from which the peptides are derived can broadly be categorized as differentiation antigens, cancer/testis antigens, mutated gene products, widely expressed proteins, viral antigens and most recently, phosphopeptides derived from dysregulated signal transduction pathways. (Zarling el al., 2006).

Immunization with cancer-derived, class I or class II MHC-encoded molecule associated peptides, or with a precursor polypeptide or protein that contains the peptide, or with a gene that encodes a polypeptide or protein containing the peptide, are forms of immunotherapy that can be employed in the treatment of colorectal cancer. Identification of the immunogens is a necessary first step in the formulation of the appropriate immunotherapeutic agent or agents. Although a large number of tumor-associated peptide antigens recognized by tumor reactive CTL have been identified, there are few examples of antigens that are derived from proteins that are selectively expressed on a broad array of tumors, as well as associated with cellular proliferation and/or transformation.

Attractive candidates for this type of antigen are peptides derived from proteins that are differentially phosphorylated on serine (Ser), threonine (Thr), and/or tyrosine (Tyr; Zarling et al., 2000). Due to the increased and dysregulated phosphorylation of cellular proteins in transformed cells as compared to normal cells, tumors are likely to present a unique subset of phosphorylated peptides on the cell surface that are available for recognition by cytotoxic T-lymphocytes (CTL). Presently, there is no way to predict which protein phosphorylation sites in a cell will be unique to tumors, survive the antigen processing pathway, and be presented to the immune system in the context of 8-14 residue phosphopeptides bound to class I MHC molecules.

Thirty-six phosphopeptides were disclosed as presented in association with HLA-A*0201 on cancer cells (see Table 1 of Zarling et al., 2006). Parent proteins for four of these peptides (β-catenin, insulin receptor substrate-2 (IRS-2), tensin-3, and Jun-C/D) are associated with cytoplasmic signaling pathways and cellular transformation.

Until the present disclosure, no studies have examined MHC class-I-bound phosphopeptide displayed on primary human tumor samples and, there is only limited evidence of a human immune response against class-I-restricted phosphopeptides.

SUMMARY

This Summary lists several embodiments of the presently disclosed subject matter, and in many cases lists variations and permutations of these embodiments. This Summary is merely exemplary of the numerous and varied embodiments. Mention of one or more representative features of a given embodiment is likewise exemplary. Such an embodiment can typically exist with or without the feature(s) mentioned; likewise, those features can be applied to other embodiments of the presently disclosed subject matter, whether listed in this Summary or not. To avoid excessive repetition, this Summary does not list or suggest all possible combinations of such features.

In some embodiments, the presently disclosed subject matter relates to compositions comprising at least or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more synthetic target peptides, each of which are about or at least 8, 9, 10, 11, 12, 13, 14, or 15 amino acids long. In some embodiments, the target peptides comprise an amino acid sequence as set forth in any of SEQ ID NOs: 1-177. In some embodiments, the composition has the ability to stimulate a T cell-mediated immune response to at least one of the synthetic target peptides.

In some embodiments, at least one serine, tyrosine, and/or threonine residue in any of the peptides is phosphorylated. In some embodiments, at least one serine residue in any of the peptides is replaced with a homoserine. In some embodiments, the composition comprises a non-hydrolyzable phosphate. In some embodiments, at least one phosphoserine residue in any of the peptides is replaced by phosphohomoserine. In some embodiments, the composition is immunologically suitable for at least 60 to 88% of colorectal cancer patients. In some embodiments, the composition comprises at least five different target peptides. In some embodiments, the composition comprises at least ten different target peptides. In some embodiments, the composition comprises at least fifteen different target peptides.

In some embodiments, the composition comprises a target peptide capable of binding to an MHC class I molecule. In some embodiments, the target peptide is capable of binding to an MHC class I molecule selected from the group consisting of HLA-A*0201, HLA-A*0101, HLA-A*0301, HLA-B*4402, HLA-B*0702, HLA-B*-2705, and HLA-B*1402. In some embodiments, the composition comprises a peptide capable of binding to an MHC class I molecule selected from the group consisting of HLA-A*0101, HLA-A*0301, HLA-B*4402, HLA-B*0702, HLA-B*-2705, and HLA-B*1402. In some embodiments, the composition comprises a peptide capable of binding to an MHC class I molecule of the HLA-A*0201, HLA-A*0101, or HLA-B*0702 alleles.

In some embodiments, the composition is capable of increasing the 5-year survival rate of colorectal cancer patients treated with the composition by at least 5%, 10%, 15%, 20%, 25%, 30%, 40%, or 50% relative to average 5-year survival rates that could have been expected without treatment with the composition. In some embodiments, the composition is capable of increasing the survival rate of colorectal cancer patients treated with the composition by at least 5%, 10%, 15%, 20%, 25%, 30%, 40%, or 50% relative to a survival rate that could have been expected without treatment with the composition. In some embodiments, the composition is capable of increasing the treatment response rate of colorectal cancer patients treated with the composition by at least 5%, 10%, 15%, 20%, 25%, 30%, 40%, or 50% relative to a treatment rate that could have been expected without treatment with the composition. In some embodiments, the composition is capable of increasing the overall median survival of patients of colorectal cancer patients treated with the composition by at least two months relative to an overall median survival that could have been expected without treatment with the composition.

In some embodiments, the composition comprises at least one peptide derived from MelanA (MART-I), gp100 (Pmel 17), tyrosinase, TRP-1, TRP-2, MAGE-1, MAGE-3, BAGE, GAGE-1, GAGE-2, p15(58), CEA, RAGE, NY-ESO (LAGE), SCP-1, Hom/Mel-40, PRAME, p53, H-Ras, HER-2/neu, BCR-ABL, E2A-PRL, H4-RET, IGH-IGK, MYL-RAR, Epstein Barr virus antigens, EBNA, human papillomavirus (HPV) antigens E6 and E7, TSP-180, MAGE-4, MAGE-5, MAGE-6, p185erbB2, p180erbB-3, c-met, nm-23H1, PSA, TAG-72-4, CA 19-9, CA 72-4, CAM 17.1, NuMa, K-ras, β-Catenin, CDK4, Mum-1, p16, TAGE, PSMA, PSCA, CT7, telomerase, 43-9F, 5T4, 791Tgp72, α-fetoprotein, β-HCG, BCA225, BTAA, CA 125, CA 15-3 (CA 27.29\BCAA), CA 195, CA 242, CA-50, CAM43, CD68\KP1, CO-029, FGF-5, G250, Ga733 (EpCAM), HTgp-175, M344, MA-50, MG7-Ag, MOV18, NB/70K, NY-CO-1, RCAS1, SDCCAG16, TA-90 (Mac-2 binding protein/cyclophilin C-associated protein), TAAL6, TAG72, TLP and TPS.

In some embodiments, the composition comprises an adjuvant. In some embodiments, the adjuvant is selected from the group consisting of montanide ISA-51 (Seppic, Inc., Fairfield, N.J., United States of America), QS-21 (Aquila Biopharmaceuticals, Inc., Lexington, Mass., United States of America), tetanus helper peptides, GM-CSF, cyclophosphamide, bacillus Calmette-Guerin (BCG), Corynbacterium parvum, levamisole, azimezone, isoprinisone, dinitrochlorobenezene (DNCB), keyhole limpet hemocyanins (KLH), Freunds adjuvant (complete and incomplete), mineral gels, aluminum hydroxide (Alum), lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, dinitrophenol, and diphtheria toxin (DT).

In some embodiments, the presently disclosed subject matter also relates to an in vitro population of dendritic cells comprising a composition comprising at least one target peptide as disclosed herein. In some embodiments, the at least one target peptide selected from the group consisting of SEQ ID NOs: 1-177.

In some embodiments, the presently disclosed subject matter also relates to an in vitro population of CD8⁺ T cells capable of being activated upon being brought into contact with a population of dendritic cells, wherein the dendritic cells comprise a composition comprising at least one target peptide as disclosed herein. In some embodiments, the at least one target peptide selected from the group consisting of SEQ ID NOs: 1-177.

In some embodiments, the presently disclosed subject matter also relates to an antibody or antibody-like molecule that specifically binds to both a first complex of MHC class I molecule and a target peptide. In some embodiments, the at least one target peptide selected from the group consisting of SEQ ID NOs: 1-177. In some embodiments, the antibody or antibody-like molecule is a member of the immunoglobulin superfamily. In some embodiments, the antibody or antibody-like molecule comprises a binding member selected from the group consisting an Fab, Fab′, F(ab′)₂, Fv, and a single-chain antibody. In some embodiments, the antibody or antibody-like molecule comprises a therapeutic agent selected from the group consisting of an alkylating agent, an antimetabolite, a mitotic inhibitor, a taxoid, a vinca alkaloid, and an antibiotic. In some embodiments, the antibody or antibody-like molecule is a T cell receptor, which in some embodiments is optionally linked to a CD3 agonist.

In some embodiments, the presently disclosed subject matter also relates to an in vitro population of T cells transfected with an mRNA encoding a target peptide-specific T cell receptor as disclosed herein.

The presently disclosed subject matter also relates in some embodiments to methods for treating and/or preventing cancer. In some embodiments, the presently disclosed methods comprise administering to a patient in need thereof one or more doses of a composition comprising at least one target peptide as disclosed herein. In some embodiments, the at least one target peptide selected from the group consisting of SEQ ID NOs: 1-177.

In some embodiments, the presently disclosed subject matter also relates to methods for treating and/or preventing colorectal cancer. In some embodiments, the presently disclosed methods comprise administering to a patient in need thereof one or more doses of a composition comprising at least one target peptide as disclosed herein, wherein the composition in some embodiments comprises a pharmaceutically acceptable carrier, which in some embodiments is pharmaceutically acceptable for use in a human. In some embodiments, the at least one target peptide selected from the group consisting of SEQ ID NOs: 1-177.

In some embodiments, the presently disclosed subject matter also relates to methods for treating and/or preventing cancer comprising administering to a patient in need thereof a dose of a CD8⁺ T cell in combination with a pharmaceutically acceptable carrier, optionally a carrier that is pharmaceutically acceptable for use in a human. In some embodiments, the CD8⁺ T cell is capable of being activated upon being brought into contact with a population of dendritic cells, and further wherein the dendritic cells comprise a composition comprising at least one target peptide as disclosed herein.

In some embodiments, the presently disclosed subject matter also relates to methods for treating and/or preventing cancer comprising administering to a patient in need thereof the population of dendritic cells comprising a composition comprising at least one target peptide as disclosed herein. In some embodiments, the dendritic cells are administered in combination with a pharmaceutically acceptable carrier, optionally a carrier that is pharmaceutically acceptable for use in a human.

The presently disclosed subject matter also relates in some embodiments to methods for treating and/or preventing cancer comprising administering to a patient in need thereof a population T cells as disclosed herein in combination with a pharmaceutically acceptable carrier, optionally a carrier that is pharmaceutically acceptable for use in a human.

The presently disclosed subject matter also relates in some embodiments to methods for making a cancer vaccine. In some embodiments, the presently disclosed methods comprise combining a composition as disclosed herein with an adjuvant and a pharmaceutically acceptable carrier and placing the composition, adjuvant, and pharmaceutical carrier into a syringe.

The presently disclosed subject matter also relates in some embodiments to methods for screening a target peptide for inclusion in an immunotherapy composition. In some embodiments, the presently disclosed methods comprise administering the target peptide to a human; determining whether the target peptide is capable of inducing a target peptide-specific memory T cell response in the human; and selecting the target peptide for inclusion in an immunotherapy composition if the target peptide elicits a target peptide-specific memory T cell response in the human.

In some embodiments, the presently disclosed subject matter also relates to methods for determining a prognosis of a cancer patient. In some embodiments, the presently disclosed methods comprise administering a target peptide associated with the patient's cancer to the patient; determining whether the target peptide is capable of inducing a target peptide-specific memory T cell response in the patient; and determining that the patient has a better prognosis if the patient mounts a memory T cell response to the target peptide than if the patient did not mount a memory T cell response to the target peptide.

In some embodiments, the presently disclosed subject matter also relates to kits comprising at least one target peptide composition as disclosed herein, wherein the at least one target peptide composition comprises at least one target peptide and a cytokine and/or an adjuvant. In some embodiments, the kit comprises at least 2, 3, 4, 5, or more target peptide compositions as disclosed herein. In some embodiments, the cytokine is selected from the group consisting of a transforming growth factor (TGF) including but not limited to TGF-α and TGF-β; insulin-like growth factor (IGF)-I; IGF-II; erythropoietin (EPO); an osteoinductive factor; an interferon (IFN) including but not limited to IFNα, IFNβ, and IFNγ; a colony stimulating factor (CSF) including but not limited to macrophage-CSF (M-CSF); granulocyte-macrophage-CSF (GM-CSF); and granulocyte-CSF (G-CSF); an interleukin (IL) including but not limited to IL-1, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12; IL-13, IL-14, IL-15, IL-16, IL-17, IL-18; leukemia inhibitory factor (LIF); kit-ligand; FLT-3; angiostatin; thrombospondin; endostatin; an tumor necrosis factor (TNF) including but not limited to TNFα and TNFβ; and lymphotoxin (LT). In some embodiments, the adjuvant selected from the group consisting of montanide ISA-51 (Seppic, Inc., Fairfield, N.J., United States of America), QS-21 (Aquila Biopharmaceuticals, Inc., Lexington, Mass., United States of America), a tetanus helper peptide, GM-CSF, cyclophosphamide, bacillus Calmette-Guerin (BCG), Corynbacterium parvum, levamisole, azimezone, isoprinisone, dinitrochlorobenezene (DNCB), keyhole limpet hemocyanin (KLH), Freunds adjuvant (complete and incomplete), a mineral gel, aluminum hydroxide (Alum), lysolecithin, a pluronic polyol, a polyanion, a peptide, an oil emulsion, dinitrophenol, and diphtheria toxin (DT).

In some embodiments, the kit comprises at least one additional peptide. In some embodiments, the at least one additional peptide is a peptide derived from MelanA (MART-I), gp100 (Pmel 17), tyrosinase, TRP-1, TRP-2, MAGE-1, MAGE-3, MAGE-4, MAGE-5, MAGE-6, BAGE, GAGE-1, GAGE-2, p15(58), CEA, RAGE, NY-ESO (LAGE), SCP-1, Hom/Mel-40, PRAME, p53, H-Ras, HER-2/neu, BCR-ABL, E2A-PRL, H4-RET, IGH-IGK, MYL-RAR, an Epstein Barr virus antigen, EBNA, human papillomavirus (HPV) antigen E6, HPV antigen E7, TSP-180, p185erbB2, p180erbB-3, c-met, nm-23H1, PSA, TAG-72-4, CA 19-9, CA 72-4, CAM 17.1, NuMa, K-ras, β-Catenin, CDK4, Mum-1, p16, TAGE, PSMA, PSCA, CT7, telomerase, 43-9F, 5T4, 791Tgp72, α-fetoprotein, β-HCG, BCA225, BTAA, CA 125, CA 15-3 (CA 27.29/BCAA), CA 195, CA 242, CA-50, CAM43, CD68/KP1, CO-029, FGF-5, G250, Ga733 (EpCAM), HTgp-175, M344, MA-50, MG7-Ag, MOV18, NB/70K, NY-CO-1, RCAS1, SDCCAG16, TA-90 (Mac-2 binding protein/cyclophilin C-associated protein), TAAL6, TAG72, TLP, and TPS.

In some embodiments, the kit comprises at least one target peptide comprising an amino acid sequence as set forth in any of SEQ ID NOs: 1-177.

In some embodiments, the presently disclosed subject matter also provides methods for inducing a target peptide-specific memory T cell response in a patient having a proliferative disorder. In some embodiments, the presently disclosed methods comprise (a) administering to a patient in need thereof a composition comprising at least one target peptide and an adjuvant; and (b) inducing a memory T cell response to the at least one target peptide. In some embodiments, the memory T cell response is capable of treating the proliferative disorder. In some embodiments, the at least one target peptide is selected from SEQ ID NOs: 1-177. In some embodiments, the proliferative disorder is cancer. In some embodiments, the cancer is colorectal cancer. In some embodiments, the adjuvant is a TLR agonist. In some embodiments, the adjuvant is a Montanide ISA-51. In some embodiments, the adjuvant is a tetanus helper peptide, a modified tetanus helper peptide (including but not limited to a peptide that is at least 90% identical to a a segment or fragment of a tetanus toxoid protein). In some embodiments, the composition comprises at least one peptide derived from MelanA (MART-I), gp100 (Pmel 17), tyrosinase, TRP-1, TRP-2, MAGE-1, MAGE-3, BAGE, GAGE-1, GAGE-2, p15(58), CEA, RAGE, NY-ESO (LAGE), SCP-1, Hom/Mel-40, PRAME, p53, H-Ras, HER-2/neu, BCR-ABL, E2A-PRL, H4-RET, IGH-IGK, MYL-RAR, an Epstein Barr virus antigen, EBNA, a human papillomavirus (HPV) antigen E6 and/or E7, TSP-180, MAGE-4, MAGE-5, MAGE-6, p185erbB2, p180erbB-3, c-met, nm-23H1, PSA, TAG-72-4, CA 19-9, CA 72-4, CAM 17.1, NuMa, K-ras, β-Catenin, CDK4, Mum-1, p16, TAGE, PSMA, PSCA, CT7, telomerase, 43-9F, 5T4, 791Tgp72, α-fetoprotein, β-HCG, BCA225, BTAA, CA 125, CA 15-3 (CA 27.29\BCAA), CA 195, CA 242, CA-50, CAM43, CD68KP1, CO-029, FGF-5, G250, Ga733 (EpCAM), HTgp-175, M344, MA-50, MG7-Ag, MOV18, NB/70K, NY-CO-1, RCAS1, SDCCAG16, TA-90 (Mac-2 binding protein/cyclophilin C-associated protein), TAAL6, TAG72, TLP, and TPS. In some embodiments, the composition is immunologically suitable for at least 60 to 88% of cancer patients. In some embodiments, the composition comprises at least 1, 2, 3, 4, or 5 different target peptides. In some embodiments, the composition comprises at least 10 different target peptides. In some embodiments, the composition comprises at least 15 different target peptides. In some embodiments, the composition comprises a peptide capable of binding to an MHC class I molecule selected from the group consisting of HLA-A*0201, HLA-A*0101, HLA-A*0301, HLA-B*4402, HLA-B*0702, HLA-B*-2705 and HLA-B*1402. In some embodiments, the composition comprises a peptide capable of binding to an MHC class I molecule selected from the group consisting of HLA-A*0101, HLA-A*0301, HLA-B*4402, HLA-B*0702, HLA-B*-2705, HLA-B*1402 and combinations thereof. In some embodiments, the composition comprises a peptide capable of binding to an MHC class I molecule of the HLA-A*0201, HLA-A*0101 or HLA-B*0702 alleles. In some embodiments, the composition comprises an agent selected from the group consisting of a CTLA-4 antagonist, vermurafenib, ipilimumab, dacarbazine, IL-2, temozolomide, imatinib, gefitinib, erlotinib, sunitinib, tyrphostins, a PD-1 agonist, and telatinib.

In some embodiments, the presently disclosed methods increase the 5-year survival rate of the patients treated with the composition by at least 20 percent relative to average 5-year survival rates that could have been expected without treatment with the composition. In some embodiments, the presently disclosed methods are capable of increasing the overall median survival of patients treated with the composition by at least two months relative to an overall median survival that could have been expected without treatment with the composition.

In some embodiments, the presently disclosed methods further comprise administering to the patient darcarbazine, carmustine, tamoxifen, or a combination thereof including but not limited to administering darcarbazine, carmustine, and tamoxifen.

In some embodiments, the presently disclosed methods further comprise administering to the patient an adjuvant selected from the group consisting of montanide ISA-51 (Seppic, Inc.), QS-21 (Aquila Pharmaceuticals, Inc.), a tetanus helper peptide, GM-CSF, cyclophosphamide, bacillus Calmette-Guerin (BCG), Corynbacterium parvum, levamisole, azimezone, isoprinisone, dinitrochlorobenezene (DNCB), keyhole limpet hemocyanins (KLH), Freunds adjuvant (complete and incomplete), mineral gels, aluminum hydroxide (Alum), lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, dinitrophenol, diphtheria toxin (DT).

In some embodiments, the presently disclosed methods further comprise determining that the T cell memory response is a T cell central memory response (T_(CM)).

These and other aspects and embodiments that will be apparent to those of skill in the art upon reading the specification provide the art with immunological tools and agents useful for diagnosing, prognosing, monitoring, and/or treating cancers including but not limited to human cancers.

BRIEF DESCRIPTION OF THE FIGURES

A more complete understanding of the presently disclosed subject matter can be obtained by reference to the accompanying Figures, when considered in conjunction with the subsequent Detailed Description. The embodiments illustrated in the Figures are intended to be exemplary only, and should not be construed as limiting the presently disclosed subject matter.

FIGS. 1A-1C illustrate various T cell responses to tumor specific phosphopeptides of the presently disclosed subject matter. FIG. 1A depicts the results of multiplexed intracellular cytokine staining for IL-2, IFNγ, and TNFα. FIG. 1B is a graph showing that multifunctional TILs responded to the phosphopeptides identified producing IL-2, IFNγ, and TNFα. In FIG. 1B, responses to IL-2/IFNγ/TNFα, TNFα alone, IFNγ and TNFα, IFNγ alone, IL-2 and IFNγ, IL-2 alone, and TNFα and IL-2 are depicted. The responses are also summarized in FIG. 1C. In FIG. 1C, white boxes indicate less than 0.5%, hatched boxes indicate 0.5-1.0%, light gray boxes indicate 1.0-2.00%, and dark gray boxes indicate greater than 2.0%.

FIG. 2 illustrates T cell responses in 13 healthy donors to a variety of CRC-associated phosphopeptides. In FIG. 2, a box with a diagonal line indicates that the corresponding phosphopeptide was not detected, a white box indicates that 5-24 spots were detected by ELISpot, a light gray box indicates that 25-99 spots were detected, and a dark gray box indicates that 100 or more spots were detected.

FIG. 3 is a series of graphs illustrating that T cells lines established against phosphopeptide targets IRS2 (HLA-A2; bottom panel) and ZPF36L2 (HLA-B7; top panel) were able to specifically kill the CRC cell line SW620 (ATCC® CCL-227™: American Type Culture Collection, Manassas, Va., United States of America).

FIG. 4 is a Table summarizing the results of a Day 7 ELISpot experiment that demonstrated that healthy humans have an immunological recall response to certain colorectal class I phosphopeptides. Importantly, this response comes from central memory T cells and is comparable to the recall response that the same individuals have to viral peptides presented as a result of flu, EBV, etc. The left column lists various synthetic samples of CRC phosphopeptides. They were tested on 8 healthy B7/A3 donors and memory T cell responses were observed for several of the peptides. “KPE” is KPEsRRSSLL (SEQ ID NO: 54); “AVR” is AVRPTRLsL (SEQ ID NO: 93); “RPD” is RPDsAHKML (SEQ ID NO: 67); “SPR” is SPRsPDRTL (SEQ ID NO: 87); “RPT” is RPTKIGRRsL (SEQ ID NO: 81); “QPQ” is QPQRRsLRL (SEQ ID NO: 63); “RPG” is RPGsRQAGL (SEQ ID NO: 71); “SPF” is SPFKRQLsL (SEQ ID NO: 84); “APD”: is APDsPRAFL (SEQ ID NO: 49); “LPI” is LPIFSRLsI (SEQ ID NO: 60); “RPR” is RPRARsVDAL (SEQ ID NO: 75); “RLS” is RLSsPISKR (SEQ ID NO: 39); “SVR” is SVRRsVLMK (SEQ ID NO: 45); “RTM” is RTMsEAALVRK (SEQ ID NO: 40); “RPF” is RPFHGISTVsL (SEQ ID NO: 69); “RQAv” is RQAsIELPSMAV (SEQ ID NO: 18); “RRG” is RRGsFEVTL (SEQ ID NO: 132); “RTH” is RTHsLLLLL (SEQ ID NO: 176); “NLV“and” TPR” are cytomegalovirus (CMV) peptides, “GLC” and “RPP” are Epstein-Barr Virus (EBV) peptides; “FLU” and “ADENO” are influenza and adenoviral peptides respectively. Phosphopeptide sequences; pSer, pThr, and pTyr are specified by lowercase s, t, and y, respectively.

FIG. 5 is a schematic diagram of the isolating and characterization of phosphopeptides present in CRC tumor samples.

FIG. 6 is two graphs showing TIL responses to phosphopeptides derived from IRS2 (left panel) and ZFP36L2 (SEQ ID NO: 60; right panel).

BRIEF DESCRIPTION OF TABLES 3-9

Tables 3-5 provide a listing of exemplary peptides including phosphopeptides of the presently disclosed subject matter.

Tables 3 and 4 are Table showing a list of selected CRC-associated phosphopeptides, predicted HLA type, presence in the liver in the form of a metastasis, and notes on the pathway with which the protein from which the phosphopeptide is derived is associated.

Table 5 is a Table listing various phosphopeptides associated with CRC.

Tables 6-8 list characteristics of HLA-DR-associated phosphopeptides selectively expressed by melanoma cells. Table 6 is a table derived from PCT International Patent Application Publication No. WO 2010/129537 that lists characteristics of HLA-DR-associated phosphopeptides selectively expressed by melanoma cells. Tables 7 and 8 are derived from Depontieu et al., 2009, including Depontieu et al. Supplemental Information, 10.1073 Proc Natl Acad Sci USA 0903852106. Table 7 lists characteristics of HLA-DR-associated phosphopeptides selectively expressed by EBV-transformed B Cells. Table 8 lists characteristics of HLA-DR-associated phosphopeptides commonly expressed by melanoma and EBV-transformed B Cells.

Table 9 lists characteristics of source proteins generating HLA-DR-restricted phosphopeptides.

DETAILED DESCRIPTION

While the following terms are believed to be well understood by one of ordinary skill in the art, the following definitions are set forth to facilitate explanation of the presently disclosed subject matter.

All technical and scientific terms used herein, unless otherwise defined below, are intended to have the same meaning as commonly understood by one of ordinary skill in the art. Mention of techniques employed herein are intended to refer to the techniques as commonly understood in the art, including variations on those techniques or substitutions of equivalent techniques that would be apparent to one of skill in the art. While the following terms are believed to be well understood by one of ordinary skill in the art, the following definitions are set forth to facilitate explanation of the presently disclosed subject matter.

Following long-standing patent law convention, the terms “a”, “an”, and “the” refer to “one or more” when used in this application, including the claims. Thus, in some embodiments the phrase “a peptide” refers to one or more peptides.

The term “about”, as used herein to refer to a measurable value such as an amount of weight, time, dose (e.g., therapeutic dose), etc., is meant to encompass in some embodiments variations of +20%, in some embodiments ±10%, in some embodiments ±5%, in some embodiments ±1%, in some embodiments ±0.1%, in some embodiments 0.5%, and in some embodiments ±0.01% from the specified amount, as such variations are appropriate to perform the disclosed methods.

As used herein, the term “and/or” when used in the context of a list of entities, refers to the entities being present singly or in any possible combination or subcombination. Thus, for example, the phrase “A, B, C, and/or D” includes A, B, C, and D individually, but also includes any and all combinations and subcombinations of A, B, C, and D.

I. Target Peptides

The presently disclosed subject matter relates in some embodiments to post-translationally modified immunogenic therapeutic target peptides, e.g., phosphopeptides and optionally phosphopeptides comprising modified amino acids including, but not limited to phosphohomoserine, for use in immunotherapy and diagnostic methods of using the target peptides, as well as methods of selecting the same to make compositions for immunotherapy, e.g., in vaccines and/or in compositions useful in adaptive cell transfer.

In some embodiments, the target peptides of the presently disclosed subject matter are post-translationally modified by being provided with a phosphate group, (i.e., “phosphopeptides” including, but not limited to peptides comprising phosphoserine, phosphothreonine, phosphotyrosine, and derivatives thereof including but not limited to phosphohomoserine).

The target peptides of the presently disclosed subject matter are in some embodiments not the entire proteins from which they are derived (i.e., are fragments and/or subsequences of larger polypeptides). They are in some embodiments from 8 to 50 contiguous amino acid residues of the native human protein. In some embodiments, they can contain exactly, about, or at least 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 amino acids. The peptides of the presently disclosed subject matter can in some embodiments also have a length that falls in the ranges of 8-10, 9-12, 10-13, 11-14, 12-15, 15-20, 20-25, 25-30, 30-35, 35-40, and 45-50 amino acids. In some embodiments, exactly, about, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 or more of the amino acid residues within a recited sequence of a target peptide is phosphorylated and/or contains a modified phosphoamino acid including, but not limited to phosphohomoserine.

Target peptides can be modified and analogs (using for example, β-amino acids, L-amino acids, N-methylated amino acids, amidated amino acids, non-natural amino acids, retro inverse peptides, peptoids, PNA, halogenated amino acids) can be synthesized that retain their ability to stimulate a particular immune response but which also gain one or more beneficial features, such as those described herein below. Thus, a particular target peptide can, for example, have use for treating and vaccinating against multiple cancer types.

Substitutions can be made in the target peptides at residues known to interact with the MHC molecule. Such substitutions can have the effect of increasing the binding affinity of the target peptides for the MHC molecule and can also increase the half-life of the target peptide-MHC complex, the consequence of which is that the substituted target peptide is a more potent stimulator of an immune response than is the original target peptide.

Additionally, in some embodiments the substitutions have no effect on the immunogenicity of the target peptide per se, but rather prolong its biological half-life and/or prevent it from undergoing spontaneous alterations which might otherwise negatively impact on the immunogenicity of the peptide.

The target peptides disclosed herein can have differing levels of immunogenicity, MHC binding, and ability to elicit CTL responses against cells displaying a native target peptide (e.g., on the surface of a tumor cell).

A phosphopeptide as disclosed herein is in some embodiments modified such that its immunogenicity and/or its binding is enhanced. In some embodiments, the modified target peptide binds to an MHC class I molecule about or at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 95, 100, 110, 125, 150, 175, 200, 225, 250, 275, 300, 350, 375, 400, 450, 500, 600, 700, 800, 1000% or more tightly than its native counterpart.

However, given the exquisite sensitivity of the T cell receptor, it cannot be foreseen whether such enhanced binding and/or immunogenicity will render a modified target peptide still capable of inducing an activated CTL that will cross react with the native target peptide being displayed on the surface of a tumor. Indeed, it is disclosed herein that the binding affinity of a target peptide does not predict its functional ability to elicit a T cell response.

Target peptides of the presently disclosed subject matter can in some embodiments be mixed together to form a cocktail. The target peptides can be in an admixture, or they can be linked together in a concatamer and/or in other arrangement as a single molecule. Linkers between individual target peptides can be used; these can, for example, be formed in some embodiments by any 10 to 20 amino acid residues. The linkers can be random sequences, or they can be optimized for degradation by dendritic cells.

In certain specified positions, a native amino acid residue in a native human protein can be altered to enhance its binding to an MHC class I molecule. These occur in “anchor” positions of the target peptides, often in positions 1, 2, 3, 9, or 10. Valine, alanine, lysine, leucine tyrosine, arginine, phenylalanine, proline, glutamic acid, 5 threonine, serine, aspartic acid, tryptophan, and methionine can also be used as improved anchoring residues. Anchor residues for different HLA molecules are listed below in Table 1.

TABLE 1 Anchor Residues for HLA Molecules HLA Type Residue Position Anchor Residue(s) HLA A*0201 2 L, M 9 or Last V HLA A*0301 2 L, M 9 or Last K HLA A*0101 2 T, S 3 D, E 9 or Last Y HLA B*2705 1 R 2 R 9 or Last L, F, K, R, M HLA B*0702 2 P 9 or Last L, M, V, F HLA B*4402 2 E 9 or Last F, Y, W

In some embodiments, the immunogenicity of a target peptide is measured using transgenic mice expressing human MHC class I genes. For example, “ADD Tg mice” express an interspecies hybrid class I MHC gene, AAD, which contains the α-1 and α-2 domains of the human HLA-A2.1 gene and the α-3 transmembrane and cytoplasmic domains of the mouse H-2Dd gene, under the transcriptional control of the human HLA-A2.1 promoter. Immunodetection of the HLA-A2.1 recombinant transgene established that expression was at equivalent levels to endogenous mouse class I molecules. The mouse α-3 domain expression enhances the immune response in this system. Compared to unmodified HLA-A2.1, the chimeric HLA-A2.1/H2-Dd MHC Class I molecule mediates efficient positive selection of mouse T cells to provide a more complete T cell repertoire capable of recognizing peptides presented by HLA-A2.1 Class I molecules.

The peptide epitopes presented and recognized by mouse T cells in the context of the HLA-A2.1/H2-Dd class I molecule are the same as those presented in HLA-A2.1⁺ humans. This transgenic strain enables the modeling of human T cell immune responses to HLA-A2 presented antigens, and identification of those antigens. This transgenic strain is a preclinical model for design and testing of vaccines for infectious diseases or cancer therapy involving optimal stimulation of CD8⁺ cytolytic T cells.

In some embodiments, the immunogenicity of a modified phosphopeptide is determined by the degree of Interferon gamma (IFNγ) and/or tumor necrosis factor-alpha (TNF-α) production of T cells from ADD Tg mice immunized with the target peptide, e.g., by immunization with target peptide pulsed bone marrow derived dendritic cells.

In some embodiments, the modified target peptides are about or at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 95, 100, 110, 125, 150, 175, 200, 225, 250, 275, 300, 350, 375, 400, 450, 500, 600, 700, 800, 1000, 1500, 2000, 2500, 3000, 4000, 5000% or more immunogenic, e.g., in terms of numbers of IFNγ- and/or TNF-α-positive (i.e., “activated”) T cells relative to numbers elicited by native target peptides in ADD Tg mice immunized with phosphopeptide-pulsed bone marrow derived dendritic cells (BMDCs). In some embodiments, the target peptides are modified target peptides. In some embodiments, the modified target peptides are able to elicit CD8⁺ T cells that are cross-reactive with the modified and the native target peptide in general and when such modified and native target peptides are complexed with MHC class I molecules in particular. In some embodiments, the CD8 T cells that are cross-reactive with the modified and the native target peptides are able to reduce tumor size by about or at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 97% or 99% in a NOD/SCID/IL-2Rγc^(−/−) knock out mouse relative to IL-2 treatment without such cross-reactive CD8⁺ T cells.

The phrase “capable of inducing a target peptide-specific memory T cell response in a patient” as used herein relates to eliciting a response from memory T cells (also referred to as “antigen-experienced T cell”), which are a subset of infection- and cancer-fighting T cells that have previously encountered and responded to their cognate antigen. Such T cells can recognize foreign invaders, such as bacteria or viruses, as well as cancer cells. Memory T cells have become “experienced” by having encountered antigen during a prior infection, having encountered cancer, or via previous vaccination. At a second encounter with the cognate antigen (e.g., by way of an initial inoculation with a target peptide of the presently disclosed subject matter), memory T cells can reproduce to mount a faster and stronger immune response than the first time the immune system responded to the invader (e.g., through the body's own consciously unperceived recognition of a target peptide being associated with diseased tissue). This behavior can be assayed in T lymphocyte proliferation assays, which can reveal exposure to specific antigens.

Memory T cells comprise two subtypes: central memory T cells (T_(CM) cells) and effector memory T cells (T_(EM) cells). Memory cells can be either CD4⁺ or CD8⁺. Memory T cells typically express the cell surface protein CD45RO. Central memory (T_(CM)) cells generally express L-selectin and CCR7, and they secrete IL-2 but not IFNγ or IL-4. Effector memory (T_(EM)) cells, however, generally do not express L-selectin or CCR7 but produce effector cytokines like IFNγ and IL-4.

A memory T cell response generally results in the proliferation of memory T cells and/or the upregulation or increased secretion of factors such as CD45RO, L-selectin, CCR7, IL-2, IFNγ, CD45RA, CD27, and/or IL-4. In some embodiments, the target peptides of the presently disclosed subject matter are capable of inducing a T_(CM) cell response associated with L-selectin, CCR7, IL-2 but not IFNγ or IL-4 expression and/or secretion. See e.g., Hamann et al., 1997. In some embodiments, a T_(CM) cell response is associated with an at least or an about 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 90%, 95%, 97%, 98°, 99%, 100%, 125%, 150%, 175%, 200%, 250%, 300%, 400%, 500%, 600%, 700%, 800%, 900%, 1000%, 1500%, 2000% or more increase in T cell CD45RO/RA, L-selectin, CCR7, or IL-2 expression and/secretion.

In some embodiments, the target peptides of the presently disclosed subject matter are capable of inducing a CD8⁺ T_(CM) cell response in a patient the first time that patient is provided the composition including the selected target peptides. As such, the target peptides of the presently disclosed subject matter can in some embodiments be referred to as “neo-antigens.” Although target peptides might be considered “self” for being derived from self-tissue, they generally are only found on the surface of cells with a dysregulated metabolism (e.g., aberrant phosphorylation), and they are likely never presented to immature T cells in the thymus. As such, these “self” antigens act are neo-antigens because they are nevertheless capable of eliciting an immune response.

In some embodiments, about or at least 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 90%, 95%, 97%, 98%, or 99% of T cells activated by particular target peptide in a particular patient sample are T_(CM) cells.

In some embodiments, a patient sample is isolated exactly, about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more days after an initial exposure to a particular target peptide and then assayed for target peptide-specific activated T cells and the proportion of T_(CM) cells thereof.

In some embodiments, the compositions of the presently disclosed subject matter are able to elicit a CD8⁺ T_(CM) cell response in at least or about 1%, 5%, 10%, 15%, 200%, 25%, 30%, 35%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 90%, 95%, 97%, 98%, or 99% of patients and/or healthy volunteers.

In some embodiments, the compositions of the presently disclosed subject matter are able to elicit a CD8⁺ T_(CM) cell response in about or at least 1%, 5%, 10%, 15%, 200%, 25%, 30%, 35%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 90%, 95%, 97%, 98%, 99% of patients and/or healthy volunteers specific, and in some embodiments the CD8⁺ T_(CM) cell response elicited is directed against all or at least or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more target peptides that are present in the composition. In some embodiments, the aforementioned T cell activation tests are done by ELISpot assay.

II. Phosphopeptides

The term “phosphopeptides” includes MHC class I- and MHC class II-specific phosphopeptides. Exemplary MHC class I phosphopeptides are set forth in Table 5, for example.

In some embodiments, the phosphopeptides contain the sequences of at least one of the MHC class I-binding peptides listed in SEQ ID NO. 1-177. Moreover, in some embodiments about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more of the serine, homoserine, threonine, or tyrosine residues within the recited sequence is phosphorylated. The phosphorylation can be with a natural phosphorylation (—CH₂—O—PO₃H) or with an enzyme non-degradable, modified phosphorylation, such as but not limited to —CH₂—CF₂—PO₃H or —CH₂—CH₂—PO₃H. Some phosphopeptides can contain more than one of the peptides listed in SEQ ID NO: 1-177, for example, if they are overlapping, adjacent, or nearby within the native protein from which they are derived.

The chemical structure of a phosphopeptide mimetic appropriate for use in the presently disclosed subject matter can in some embodiments closely approximate the natural phosphorylated residue which is mimicked, and also be chemically stable (e.g., resistant to dephosphorylation by phosphatase enzymes). This can be achieved with a synthetic molecule in which the phosphorous atom is linked to the amino acid residue, not through oxygen, but through carbon. In some embodiments, a CF₂ group links the amino acid to the phosphorous atom. Mimetics of several amino acids which are phosphorylated in nature can be generated by this approach. Mimetics of phosphoserine, phosphothreonine, and phosphotyrosine can be generated by placing a CF₂ linkage from the appropriate carbon to the phosphate moiety. The mimetic molecule L-2-amino-4 (diethylphosphono)-4, 4-difluorobutanoic acid (F2Pab) can substitute for phosphoserine (Otaka et al., 1995). L-2-amino-4-phosphono-4, 4-difluoro-3-methylbutanoic acid (F2Pmb) can substitute for phosphothreonine, and L-2-amino-4-phosphono (difluoromethyl) phenylalanine (F2Pmp) can substitute for phosphotyrosine (Smyth et al., 1992; Akamatsu et al., 1997). Alternatively, the oxygen bridge of the natural amino acid can be replaced with a methylene group. In some embodiments, serine and threonine residues are substituted with homoserine and homothreonine residues, respectively. A phosphomimetic may also include vanadate, pyrophosphate or fluorophosphates.

IRS-2 over-expression either at the gene or protein level, is evident in many different cancer types, and has been demonstrated to cause mammary tumorigenesis and enhanced metastasis in vivo. IRS proteins are adapter proteins that link signaling from upstream activators to multiple downstream effectors to modulate normal growth, metabolism, survival and differentiation. It is disclosed herein that phosphorylated IRS-2 is broadly displayed on multiple cancer types and the resulting phosphopeptide is endogenously processed and presented at levels that allow strong immune responses to be generated against it. Phosphopeptide-specific CD8+ T cells can be generated from HLA-A2 transgenic mice upon immunization with the pIRS2 phosphopeptide and these T cells are capable of recognizing and killing human melanoma and breast tumors in vitro and controlled tumor growth in a xenograft tumor model system.

Tensin 3 (TNS3) plays a role in actin remodeling. It is involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. TN3 also seems to be involved in mammary cell migration and may be involved in cell migration and bone development. The Tensin 3 phosphopeptide (VMIGsPKKV, SEQ ID NO: 27) of the presently disclosed subject matter has been surprisingly discovered to be capable of inducing a strong phosphopeptide-specific memory T cell response in a patient. This further supports the position that peptide/MHC binding affinity does not correlate with the ability of a phosphopeptide to induce a phosphopeptide specific immune response.

III. Immunosuitablity

In some embodiments, the target peptides are combined into compositions that can be used in vaccine compositions for eliciting anti-tumor immune responses and/or in adoptive T cell therapy of colorectal cancer patients. Table 5 provides phosphopeptides presented on the surface of cancer cells.

Although individuals in the human population display hundreds of different HLA alleles, some are more prevalent than others. For example, 88% of colorectal cancer patients carry at least one of the six HLA alleles: HLA-A*0201 (51%), HLA-A*0101 (29%), HLA-A*0301 (21%), HLA-B*4402 (27%), HLA-B*0702 (30%), and HLA-B*-2705 (7%).

The presently disclosed subject matter provides in some embodiments target peptides which are immunologically suitable for each of the foregoing HLA alleles. “Immunologically suitable” means that a target peptide will bind at least one allele of an MHC class I molecule in a given patient. Compositions of the presently disclosed subject matter are in some embodiments immunologically suitable for a patient when at least one target peptide of the composition will bind at least one allele of an MHC class I molecule in a given patient. Compositions of multiple target peptides presented by each of the most prevalent alleles used in a cocktail ensures coverage of the human population and to minimize the possibility that the tumor will be able to escape immune surveillance by down-regulating expression of any one class I target peptide.

The compositions of the presently disclosed subject matter can in some embodiments comprise at least one target peptide specific for one or more of the following alleles: HLA-A*0201, HLA-A*0101, HLA-A*0301, HLA-B*4402, HLA-B*0702, HLA-B*-2705, and HLA-B*1402. The compositions of the presently disclosed subject matter can in some embodiments have at least one target peptide specific for one or more of the following alleles HLA-A*0201, HLA-A*0101, HLA-A*0301, HLA-B*4402, and HLA-B*0702. Alternatively, the compositions of the presently disclosed subject matter can in some embodiments have at least one target peptide specific for HLA-A*0201, HLA-A*0101, HLA-A*0301, HLA-B*4402, HLA-B*0702, HLA-B*-2705, HLA-B*1402, or any combination thereof. The compositions may have at least one phosphopeptide specific for about or at least 1, 2, 3, 4, 5, or all 6 of the aforementioned alleles.

As such, the compositions of the presently disclosed subject matter containing various combinations of target peptides are in some embodiments immunologically suitable for between or about 3-88%, 80-89%, 70-79%, 60-69%, 57-59%, 55-57%, 53-55% or 51-53% or 5-90%, 10-80%, 15-75%, 20-70%, 25-65%, 30-60%, 35-55% or 40-50% of the population of a particular cancer including, but not limited to colorectal cancer. In some embodiments, the compositions of the presently disclosed subject matter are able to act as vaccine compositions for eliciting anti-tumor immune responses and/or in adoptive T cell therapy of colorectal cancer patients wherein the compositions are immunologically suitable for about or at least 88, 87, 86, 85, 84, 83, 82, 81, 80, 79, 78, 77, 76, 75, 74, 73, 72, 71, 70, 69, 68, 67, 66, 65, 64, 63, 62, 61, 60, 59, 58, 57, 56, 55, 54, 53, 52, 51, 50, 49, 48, 47, 46, 45, 44, 43, 42, 41, 40, 39, 38, 37, 36, 35, 34, 33, 32, 31, 30, 29, 28, 27, 26, 25, 24, 23, 22, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, or 3 percent of cancer such as, but not limited to colorectal cancer patients.

IV. Compositions

The phrase “target peptide compositions” as used herein refers to at least one target peptide formulated, for example, as a vaccine; or as a preparation for pulsing cells in a manner such that the pulsed cells, e.g., dendritic cells, will display the at least one target peptide in the composition on their surface, e.g., to T cells in the context of adoptive T cell therapy.

The compositions of the presently disclosed subject matter can in some embodiments include about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 50-55, 55-65, 65-80, 80-120, 90-150, 100-175, or 175-250 different target peptides.

The compositions of the presently disclosed subject matter in some embodiments generally include MHC class I specific target peptide(s) but can also include one or more target peptides specific for MHC class 11, such as but not limited to the peptides set forth in Tables 6-9, or other peptides associated with tumors (e.g., tumor associated antigen (“TAA”)).

Compositions comprising the target peptide are typically substantially free of other human proteins or peptides. They can be made synthetically or by purification from a biological source. They can be made recombinantly. Desirably they are in some embodiments at least 90% pure, in some embodiments at least 92% pure, in some embodiments at least 93% pure, in some embodiments at least 94% pure, in some embodiments at least 95% pure, in some embodiments at least 96% pure, in some embodiments at least 97% pure, in some embodiments at least 98% pure, and in some embodiments at least 99% pure. For administration to a human, they generally do not contain other components that might be harmful to a human recipient (referred to herein as “pharmaceutically acceptable for use in a human”). The compositions are typically devoid of cells, both human and recombinant producing cells. However, as noted below, in some cases, it can be desirable to load dendritic cells with a target peptide and use those loaded dendritic cells as either an immunotherapy agent themselves or as a reagent to stimulate a patient's T cells ex vivo. The stimulated T cells can be used as an immunotherapy agent.

In some cases, it can be desirable to form a complex between a target peptide and an HLA molecule of the appropriate type. Such complexes can be formed in vitro or in vivo. Such complexes are in some embodiments tetrameric with respect to an HLA-target peptide complex.

In some embodiments, the target peptide of the presently disclosed subject matter comprises an amino acid sequence that has at least 50, 60, 70, 80, 90, 95, 98, 99 or 100 percent sequence homology to an amino acid sequence as set forth in any of SEQ ID NOs: 1-177. As used herein, the phrase “sequence homology” refers to the presence of homology between two amino acid sequences. The “percentage of sequence homology” can be determined by comparing two optimally aligned sequences over a comparison window (e.g., about 5-25 contiguous amino acids or more), wherein the portion of the amino acid sequence in the comparison window can include additions or deletions (i.e., gaps) as compared to a reference sequence for optimal alignment of the two sequences. Optimal alignment of sequences for comparison can be conducted by computerized implementations of known algorithms, or by visual inspection. Readily available sequence comparison and multiple sequence alignment algorithms are, respectively, the Basic Local Alignment Search Tool (BLAST; Altschul et al., 1990; Altschul et al., 1997) and ClustalX (Chenna et al., 2003) programs, both available on the Internet. Other suitable programs include, but are not limited to, GAP, BestFit, PlotSimilarity, and FASTA, which are part of the Accelrys GCG Package available from Accelrys Software, Inc. of San Diego, Calif., United States of America.

Under certain circumstances it can be desirable to add additional proteins or peptides, for example, to make a cocktail having the ability to stimulate an immune response in a number of different HLA type hosts. Alternatively, additional proteins and/or peptides can provide an interacting function within a single host, such as but not limited to an adjuvant function or a stabilizing function. As a non-limiting example, other tumor antigens can be used in admixture with the target peptides such that multiple different immune responses are induced in a single patient.

Administration of target peptides to a mammalian recipient can be accomplished using long target peptides (e.g., longer than 15 residues), and/or using target peptide-loaded dendritic cells. See Melief, 2009. In some embodiments, an immediate goal of the administration of target peptides is to induce activation of CD8⁺ T cells in a subject. Additional components that can be administered to the same subject, either at the same time and/or close in time (such as but not limited to within 3, 5, 7, 10, 14, 17, or 21 days of each other, or even longer) include TLR-ligand oligonucleotide CpG and related target peptides that have overlapping sequences of at least six amino acid residues. To ensure efficacy, mammalian recipients should express the appropriate human HLA molecules to bind to the target peptides. Transgenic mammals can be used as recipients, for example, if they express appropriate human HLA molecules. If a mammal's own immune system recognizes a similar target peptide then it can be used as model system directly without introducing a transgene. Useful models and recipients can be at increased risk of developing metastatic cancer, such as metastatic colorectal cancer. Other useful models and recipients can be predisposed, e.g., genetically and/or environmentally, to develop colorectal cancer or other cancer.

IV.A. Selection of Target Peptides

Disclosed herein is the finding that immune responses can be generated against phosphorylated peptides tested in healthy and diseased individuals. The T cells associated with these immune responses, when expanded in vitro, are able to recognize and kill malignant tissue (both established cells lines and primary tumor samples). Cold-target inhibition studies reveal that these target peptide-specific T cell lines kill primary tumor tissue in a target peptide-specific manner.

When selecting target peptides of the presently disclosed subject matter for inclusion in immunotherapy, e.g., in adaptive cell therapy or in the context of a vaccine, one can in some embodiments pick target peptides using one or more of the following criteria: 1) peptides associated with a particular cancer/tumor cell type; 2) a peptide derived from a gene and/or a protein associated with cell proliferation, transformation, and/or malignancy; 3) a peptide that is specific for an HLA allele carried the group of patients to be treated; and/or 4) a peptide that is capable of inducing a target peptide-specific memory T cell response in the patients to be treated upon a first exposure to a composition including the selected target peptides.

IV.B. Target Peptide Vaccines

The antigen target peptides can also be employed in a composition designed to vaccinate an individual. The antigen target peptides can in some embodiments be injected alone and can in some embodiments be administered in combination with an adjuvant and/or a pharmaceutically acceptable carrier. Vaccines are envisioned to prevent and/or treat certain diseases in general, and cancers in particular.

The target peptide-containing compositions of the presently disclosed subject matter can in some embodiments be used as a vaccine for cancer, and more specifically for melanoma, leukemia, ovarian, breast, colorectal, or lung squamous cancer, sarcoma, renal cell carcinoma, pancreatic carcinomas, squamous tumors of the head and neck, brain cancer, liver cancer, prostate cancer, ovarian cancer, and cervical cancer. The compositions can include target peptides. The vaccine compositions can in some embodiments include only the target peptides, or peptides disclosed herein, or they can include other cancer antigens that have been identified.

The vaccine compositions of the presently disclosed subject matter can be used prophylactically for the purposes of preventing, reducing the risk of, and/or delaying initiation of a cancer in an individual that does not currently have cancer. Alternatively, they can be used to treat an individual that already has cancer, so that recurrence or metastasis is delayed and/or prevented. Prevention relates to a process of prophylaxis in which the individual is immunized prior to the induction or onset of cancer. For example, in some embodiments individuals with a history of poor life style choices and at risk for developing colorectal cancer, might be immunized prior to the onset of the disease.

Alternatively, individuals that already have cancer can be immunized with the target peptide-containing compositions of the presently disclosed subject matter so as to stimulate an immune response that would be reactive against the cancer. A clinically relevant immune response would be one in which the cancer partially or completely regresses and is eliminated from the patient, and it would also include those responses in which the progression of the cancer is blocked without being eliminated. Similarly, prevention need not be total, but may result in a reduced risk, delayed onset, or delayed progression or metastasis.

The target peptide vaccines of the presently disclosed subject matter can be given to patients in some embodiments before, in some embodiments after, and/or in some embodiments during any stage of colorectal cancer. In some embodiments, they are given to patients with malignant colorectal cancer.

In some embodiments, the 5-year survival rate of patients treated with the vaccines of the presently disclosed subject matter is increased by a statistically significant amount that is, in some embodiments, by about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 or more percent, relative to the average 5-year survival rates described above.

In some embodiments, the target peptide vaccine composition will increase survival rates in patients with metastatic colorectal cancer by a statistically significant amount of time that is, in some embodiments, by about or at least, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 1.75, 2.0, 2.25, 2.5, 2.75, 3.0, 3.25, 3.5, 4.0, 4.25, 4.5, 4.75, 5.0, 5.25, 5.5, 5.75, 6.0, 6.25, 6.5, 6.75, 7.0, 7.25, 7.5, 7.75, 8.0, 8.25, 8.5, 8.75, 9.0, 9.25, 9.50, 9.75, 10.0, 10.25, 10.5, 10.75, 11.0, 11.25, 11.5, 11.75 or 12 months or more compared to what could have been expected without vaccine treatment.

In some embodiments, the survival rate (e.g., the 1, 2, 3, 4, or 5-year survival rate) of patients treated with the vaccines of the presently disclosed subject matter are increased by a statistically significant amount that is, in some embodiments, by about, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100 percent, or even greater than 100 percent, relative to the average 5-year survival rates described above.

The target peptide vaccines of the presently disclosed subject matter are in some embodiments envisioned to illicit a T cell-associated immune response such as, but not limited to generating activated CD8 T cells specific for native target peptide/MHC class I expressing cells. In some embodiments, the CD8⁺ T cells specific for native target peptide/MHC class I expressing cells are specific for at least or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more of the target peptides in the vaccine in a patient for about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100 or more days after providing the vaccine to the patient.

In some embodiments, the treatment response rates of patients treated with the target peptide vaccines of the presently disclosed subject matter are increased by a statistically significant amount that is, in some embodiments by about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 150, 200, 250, 300, 350, 400, 450, or 500 or more percent, relative to treatment without the vaccine.

In some embodiments, overall median survival of patients treated with the target peptide vaccines of the presently disclosed subject matter are increased by a statistically significant amount that is, in some embodiments, by about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 150, 200, 250, 300, 350, 400, 450, or 500 or more percent, relative to treatment without the vaccine. Preferably, the overall median survival of colorectal cancer patients treated the target peptide vaccines is envisioned to be about or at least 10.0, 10.25, 10.5, 10.75, 11.0, 11.25, 11.5, 11.75, 12, 12.25, 12.5, 12.75, 13, 13.25, 13.5, 13.75, 14, 14.25, 14.5, 14.75, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36 or more months.

In some embodiments, tumor size of patients treated with the target peptide vaccines of the presently disclosed subject matter is decreased by a statistically significant amount that is, in some embodiments, by about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 150, 200, 250, 300, 350, 400, 450, or 500 or more percent, relative to treatment without the vaccine.

In some embodiments, the compositions of the presently disclosed subject matter provide an clinical tumor regression by a statistically significant amount such as, but not limited to about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 150, 200, 250, 300, 350, 400, 450, or 500 or more percent, relative to treatment without the vaccine.

In some embodiments, the compositions of the presently disclosed subject matter provide a CTL response specific for the cancer being treated (e.g., colorectal cancer) by a statistically significant amount such as, but not limited to about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 percent of patients treated with the composition.

In some embodiments, the compositions of the presently disclosed subject matter provide an increase in progression free survival in the cancer being treated, such as but not limited to colorectal cancer, of about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500 or more weeks or months compared to the progression free survival or patients not treated with the composition.

In some embodiments, one or more of progression free survival, CTL response rates, clinical tumor regression rates, tumor size, survival rates (such as but not limited to overall survival rates), and/or response rates are determined, assessed, calculated, and/or estimated weekly, monthly, bi-monthly, quarterly, semi-annually, annually, and/or bi-annually over a period of about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more years or about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500 or more weeks.

IV.C. Compositions for Priming T Cells

Adoptive cell transfer (ACT) is the passive transfer of cells, in some embodiments immune-derived cells, into a recipient host with the goal of transferring the immunologic functionality and characteristics into the host. Clinically, this approach has been exploited to transfer either immune-promoting or tolerogenic cells (often lymphocytes) to patients to enhance immunity against cancer. The adoptive transfer of autologous tumor infiltrating lymphocytes (TIL) or genetically redirected peripheral blood mononuclear cells has been used to successfully treat patients with advanced solid tumors, including melanoma and colorectal carcinoma, as well as patients with CD19-expressing hematologic malignancies. In some embodiments, ACT therapies achieve T cell stimulation ex vivo by activating and expanding autologous tumor-reactive T cell populations to large numbers of cells that are then transferred back to the patient. See Gattinoni et al., 2006.

The target peptides of the presently disclosed subject matter can in some embodiments take the form of antigenic peptides formulated in a composition added to autologous dendritic cells and used to stimulate a T helper cell or CTL response in vitro. The in vitro generated T helper cells or CTL can then be infused into a patient with cancer (Yee et al., 2002), and specifically a patient with a form of cancer that expresses one or more of antigenic target peptides.

Alternatively, the target peptides can be added to dendritic cells (DCs) in vitro to produce loaded DCs, with the loaded DCs being subsequently transferred into an individual with cancer in order to stimulate an immune response. Alternatively, the loaded DCs can be used to stimulate CD8⁺ T cells ex vivo with subsequent reintroduction of the stimulated T cells to the patient. Although a particular target peptide might be identified on one particular cancer cell type, it might also be found on other cancer cell types.

The presently disclosed subject matter envisions treating cancer by providing a patient with cells pulsed with a composition of target peptides. The use of DCs pulsed with target peptides peptide antigens enables manipulation of the immunogen in two ways: varying the number of cells injected and varying the density of antigen presented on each cell. Exemplary non-limiting methods for DC-based based treatments can be found, for example in Mackensen et at, 2000.

IV.D. Additional Peptides Present in Target Peptide Compositions

The target peptide compositions (or target peptide composition kits comprising the same) of the presently disclosed subject matter can in some embodiments also include at least one additional peptide derived from one or more tumor-associated antigens (TAAs). Examples of TAAs include MelanA (MART-I), gp100 (Pmel 17), tyrosinase, TRP-1, TRP-2, MAGE-1, MAGE-3, BAGE, GAGE-1, GAGE-2, p15(58), CEA, RAGE, NY-ESO (LAGE), SCP-1, Hom/Mel-40, PRAME, p53, H-Ras, HER-2/neu, BCR-ABL, E2A-PRL, 4-RET, IGH-IGK, MYL-RAR, Epstein Barr virus antigens, EBNA, human papillomavirus (HPV) antigens E6 and E7, TSP-180, MAGE-4, MAGE-5, MAGE-6, p185erbB2, p180erbB-3, c-met, nm-23H1, PSA, TAG-724, CA 19-9, CA 72-4, CAM 17.1, NuMa, K-ras, β-Catenin, CDK4, Mum-1, p16, TAGE, PSMA, PSCA, CT7, telomerase, 43-9F, 5T4, 791Tgp72, α-fetoprotein, β-HCG, BCA225, BTAA, CA 125, CA 15-3 (CA27.29\BCAA), CA 195, CA 242, CA-50, CAM43, CD68\KP1, CO-029, FGF-5, G250, Ga733 (EpCAM), HTgp-275, M344, MA-50, MG7-Ag, MOV18, NB/70K, NY-CO-1, RCAS1, SDCCAG16, TA-90 (Mac-2 binding protein/cyclophilin C-associated protein), TAAL6, TAG72, TLP, TPS, prostatic acid phosphatase, and the like. Exemplary, non-limiting peptides derived from TAAs that can be incorporated into target peptide compositions (or target peptide composition kits comprising the same) of the presently disclosed subject matter are presented in Table 2.

TABLE 2 Tumor-associated Antigen¹ Peptide Sequence² gp100₂₈₀₋₂₈₈ YLEPGPVTA (SEQ ID NO: 178) Tyr₃₆₉₋₃₇₇ DYMDGTMSQV (SEQ ID NO: 179) gp100_(l7-25) ALLAVGATK (SEQ ID NO: 180) Tyr₂₄₃₋₂₅₁ KCDICTDEY (SEQ ID NO: 181) TAG-1, 2 RLSNRLLLR (SEQ ID NO: 182) — SQNFPGSQK (SEQ ID NO: 183) gp100_(l54-162) KTWGQYWQV (SEQ ID NO: 184) gp100₂₀₉₋₂₁₇ I(T/M)DQVPFSV (SEQ ID NO: 185) gp100₄₇₆₋₄₈₅ VLYRYGSFSV (SEQ ID NO: 186) MART-1/MelanA₂₇₋₃₅ AAGIGDLTV (SEQ ID NO: 187) gp100 ALNFPGSQK (SEQ ID NO: 188) gp100₆₁₄₋₆₂₂ LIYRRRLMK (SEQ ID NO: 189) NY-ESO-1 AAQERRVPR (SEQ ID NO: 190) NY-ESO-1₅₃₋₆₂ ASGPGGGAPR (SEQ ID NO: 191) NY-ESO-1 LLGPGRPYR (SEQ ID NO: 192) Tyr₂₄₀₋₂₅₁ SDAEKSDICTDEY (SEQ ID NO: 193) Tyr₁₄₆₋₁₅₆ SSDYVIPIGTY (SEQ ID NO: 194) MAGE-A1₁₆₁₋₁₆₉ EADPTGHSY (SEQ ID NO: 195) MAGE-A3₁₆₈₋₁₇₆ EVDPIGHLY (SEQ ID NO: 196) gp100₂₀₉₋₂₁₇ IMDQVPFSV (SEQ ID NO: 197) MAGE-A10₂₅₄₋₂₆₂ GLYDGMEHL (SEQ ID NO: 198) Tyr₅₆₋₇₀ AQNILLSNAPLGPQFP (SEQ ID NO: 199) Tyr₃₈₈₋₄₀₆ FLLHHAFVDSI FEQWLQRHRP (SEQ ID NO: 200) Melan-A/MART-1₅₁₋₇₃ RNGYRALMDKSL HVGTQCALTRR (SEQ ID NO: 201) MAGE-A3₂₈₁₋₂₉₅ TSYVKVLHHMVKISG (SEQ ID NO: 202) MAGE-A1, 2, 3, 6₁₂₁₋₁₃₄ LLKYRAREPVTKAE (SEQ ID NO: 203) Gp100₄₄₋₅₉ WNRQLYPEWTEAQRLD (SEQ ID NO: 204) p2₈₃₀₋₈₄₄ AQYIKANSKFIGITEL (SEQ ID NO: 205) Her2/neu₃₆₉₋₃₇₇ KIFGSLAFL (SEQ ID NO: 206) CEA₅₇₁₋₅₇₉ YLSGADLNL (SEQ ID NO: 207) Her2/neu₇₅₄₋₇₆₂ VLRENTSPK (SEQ ID NO: 208) FBP₁₉₁₋₁₉₉ EIWTHSYKV (SEQ ID NO: 209) MAGE-A1₉₆₋₁₀₄ SLFRAVITK (SEQ ID NO: 210) CEA₂₇₋₃₅ HLFGYSWYK (SEQ ID NO: 211) MART-1₉₇₋₁₁₆ APPAYEKLS (SEQ ID NO: 212) MART-1₉₈₋₁₀₉ PPAYEKLSA (SEQ ID NO: 213) MART-1₉₉₋₁₁₀ PAYEKLSAE (SEQ ID NO: 214) MART-1₉₇₋₁₁₆ VPNAPPAYEK LsAEQSPPPY (SEQ ID NO: 215) MART-1₉₈₋₁₀₉ PNAPPAYEKLsA (SEQ ID NO: 216) MART-1₉₉₋₁₁₀ NAPPAYEKLsAE (SEQ ID NO: 217) MART-1₁₀₀₋₁₁₁ APPAYEKLsAEQ (SEQ ID NO: 218) MART-1₁₀₀₋₁₁₄ APPAYEKLsAEQSPP (SEQ ID NO: 219) MART-1₁₀₀₋₁₁₅ APPAYEKLsAEQSPPP (SEQ ID NO: 220) MART-1₁₀₁₋₁₁₂ PPAYEKLsAEQS (SEQ ID NO: 221) MART-1₁₀₂₋₁₁₃ PAYEKLsAEQSP (SEQ ID NO: 222) MART-1₁₀₃₋₁₁₄ AYEKLsAEQSPP (SEQ ID NO: 223) MART-1₁₀₄₋₁₁₅ YEKLSAEQSPPP (SEQ ID NO: 224) MART-1₁₀₀₋₁₁₅ APPAYEKLSAEQSPPP (SEQ ID NO: 225) MART-1₁₀₀₋₁₁₆ APPAYEKLsAEQSPPPY (SEQ ID NO: 226) ¹the numbers listed in subscript denote the amino acid positions of the peptide sequences for each TAA. ²lowercase letters (s, t, or y) indicate positions of phosphorylation.

Such tumor-specific peptides (including the MHC class II phosphopeptides 5 disclosed in Tables 6-9) can be added to the target peptide compositions in a manner, number, and in an amount as if they were an additional target peptide added to the target peptide compositions as described herein.

IV.E. Combination Therapies

In some embodiments, the target peptide compositions (or target peptide composition kits) of the presently disclosed subject matter are administered as a vaccine or in the form of pulsed cells as first, second, third, or fourth line treatment for the cancer. In some embodiments, the compositions of the presently disclosed subject matter are administered to a patient in combination with one or more therapeutic agents. Exemplary, non-limiting therapeutic agents include anti-Programed Death-1 (PD1) or PD1-antagonists such as the anti-PD1 antibody BMS-936558 (Bristol-Myers Squibb Co., New York, N.Y., United States of America); anti-CTLA-4 or CTLA-4 antagonists; vermurafenib; ipilimumab; Dacarbazine; IL-2; Temozolomide; receptor tyrosine kinase inhibitors, including but not limited to imatinib, gefitinib, erlotinib, sunitinib, tyrphostins, telatinib; sipileucel-T; a platinum-based agent; a taxane; an alkylating agent; an antimetabolite and/or a vinca alkaloid; and combinations thereof.

In some embodiments, the cancer is sensitive to and/or refractory, relapsed, and/or resistant to one or more chemotherapeutic agents such as, but not limited to a platinum-based agent, a taxane, an alkylating agent, an anthracycline (e.g., doxorubicin including but not limited to liposomal doxorubicin), an antimetabolite, and/or a vinca alkaloid. In some embodiments, the cancer is an ovarian cancer, and the ovarian cancer is refractory, relapsed, or resistant to a platinum-based agent (e.g., carboplatin, cisplatin, oxaliplatin), a taxane (e.g., paclitaxel, docetaxel, larotaxel, cabazitaxel), and/or an anthracycline (e.g., doxorubicin including but not limited to liposomal doxorubicin). In some embodiments, the cancer is colorectal cancer, and the cancer is refractory, relapsed, or resistant to an antimetabolite (e.g., an antifolate (e.g., pemetrexed, floxuridine, raltitrexed) a pyrimidine analogue (e.g., capecitabine, cytrarabine, gemcitabine, 5FU)), and/or a platinum-based agent (e.g., carboplatin, cisplatin, irinoptecan, oxaliplatin). In some embodiments, the cancer is lung cancer, and the cancer is refractory, relapsed, or resistant to a taxane (e.g., paclitaxel, docetaxel, larotaxel, cabazitaxel), a platinum-based agent (e.g., carboplatin, cisplatin, oxaliplatin), a vinca alkaloid (e.g., vinblastine, vincristine, vindesine, vinorelbine), a vascular endothelial growth factor (VEGF) pathway inhibitor, an epidermal growth factor (EGF) pathway inhibitor) and/or an antimetabolite (e.g., an antifolate including but not limited to pemetrexed, floxuridine, or raltitrexed), and a pyrimidine analogue (e.g., capecitabine, cytrarabine, gemcitabine, 5FU). In some embodiments, the cancer is breast cancer, and the cancer is refractory, relapsed, or resistant to a taxane (e.g., paclitaxel, docetaxel, larotaxel, cabazitaxel), a VEGF pathway inhibitor, an anthracycline (e.g., daunorubicin, doxorubicin including but not limited to liposomal doxorubicin, epirubicin, valrubicin, idarubicin), a platinum-based agent (e.g., carboplatin, cisplatin, oxaliplatin), and/or an antimetabolite (e.g., an antifolate including but not limited to pemetrexed, floxuridine, or raltitrexed), and a pyrimidine analogue (e.g., capecitabine, cytrarabine, gemcitabine, 5FU). In some embodiments, the cancer is gastric cancer, and the cancer is refractory, relapsed, or resistant to an antimetabolite (e.g., an antifolate including but not limited to pemetrexed, floxuridine, raltitrexed) and a pyrimidine analogue (e.g., capecitabine, cytrarabine, gemcitabine, 5FU) and/or a platinum-based agent (e.g., carboplatin, cisplatin, oxaliplatin).

In some embodiments, the cancer is colorectal cancer. In some embodiments, chemotherapy for colorectal cancer comprises treatment with 5FU, capecitabine, irinoptecan, and/or oxaliplatin. In some embodiments, a combination of chemotherapeutics is employed. Exemplary, non-limiting chemotherapeutics that can be employed combination therapies include FOLFOX (5FU, leucovorin, and oxaliplatin); FOLFIRI (5FU, leucovorin, and irinotecan); FOLFOXIRI (leucovorin, 5FU, oxaliplatin, and irinotecan); CapeOx (capecitabine and oxaliplatin); 5FU and leucovorin; and/or capecitabine.

In some embodiments, the target peptide compositions (or target peptide composition kits comprising the same) of the presently disclosed subject matter are associated with agents that inhibit T cell apoptosis or anergy thus potentiating a T cell response (referred to herein as a “T cell potentiator”). Such agents include B7RP1 agonists, B7-H3 antagonists, B7-H4 antagonists, HVEM antagonists, HVEM antagonists, GAL9 antagonists or alternatively CD27 agonists, OX40 agonists, CD137 agonists, BTLA agonists, ICOS agonists, CD28 agonists, or soluble versions of PDL1, PDL2, CD80, CD96, B7RP1, CD137L, OX40 or CD70. See Pardoll, 2012.

In some embodiments, the T cell potentiator is a PD1 antagonist. Programmed death 1 (PD1) is a key immune checkpoint receptor expressed by activated T cells, and it mediates immunosuppression. PD1 functions primarily in peripheral tissues, where T cells can encounter the immunosuppressive PD1 ligands PD-L1 (B7-H1) and PD-L2 (B7-DC), which are expressed by tumor cells, stromal cells, or both. In some embodiments, the anti-PD1 monoclonal antibody BMS-936558 (also known as MDX-1106 and ONO-4538; Bristol-Myers Squibb) is used. In some embodiments, the T cell potentiator (e.g., PD1 antagonist) is administered as an intravenous infusion at least or about every 1, 1.5, 2, 2.5, 3, 3.5, or 4 weeks of each 4, 5, 6, 7, 8, 9, or 10-week treatment cycle of about for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or more cycles. Exemplary, non-limiting doses of the PD1 antagonists are in some embodiments exactly, about, or at least 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or more mg/kg. See Brahmer et al., 2012.

The exemplary therapeutic agents listed herein above are envisioned to be administered at a concentration of in some embodiments about 1 to 100 mg/m², in some embodiments about 10 to 80 mg/m², and in some embodiments about 40 to 60 mg/m². Further exemplary dosages include, but are not limited to about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, or more mg/m². Alternatively, an exemplary dosage range can be in some embodiments about or at least 0.001 to 100 mg/kg, in some embodiments about or at least 0.1 to 1 mg/kg, and in some embodiments about or at least 0.01 to 10 mg/kg.

The target peptide compositions (or target peptide composition kits) of the presently disclosed subject matter can in some embodiments be co-administered with cytokines such as lymphokines, monokines, growth factors, and traditional polypeptide hormones. Exemplary cytokines are growth hormones including but not limited to human growth hormone, N-methionyl human growth hormone, and bovine growth hormone; parathyroid hormone; thyroxine; insulin; proinsulin; relaxin; prorelaxin; glycoprotein hormones including but not limited to follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH), and luteinizing hormone (LH); hepatic growth factor; prostaglandin, fibroblast growth factor; prolactin; placental lactogen, OB protein; TNF-α and TNF-β; mullerian-inhibiting substance; mouse gonadotropin-associated peptide; inhibin; activin; VEGF; integrin; thrombopoietin (TPO); nerve growth factors including but not limited to NGF-β; platelet-growth factor; transforming growth factors (TGFs) including but not limited to TGF-α and TGF-β; insulin-like growth factor (IGF)-I and IGF-II; erythropoietin (EPO); osteoinductive factors; interferons (IFN) including but not limited to IFNα, IFNβ, and IFNγ; colony stimulating factors (CSFs) including but not limited to macrophage-CSF (M-CSF), granulocyte-macrophage-CSF (GM-CSF), and granulocyte-CSF (G-CSF); interleukins (ILs) including but not limited to IL-1, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, I-9, IL-10, IL-11, IL-12; IL-13, IL-14, IL-15, IL-16, IL-17, and IL-18 leukemia inhibitory factor (LIF), kit-ligand; FLT-3; angiostatin; thrombospondin; endostatin; and lymphotoxin (LT). As used herein, the term cytokine includes proteins from natural sources and/or from recombinant cell culture and biologically active equivalents thereof.

The target peptide compositions of the presently disclosed subject matter can in some embodiments be provided with administration of cytokines around the time of (including but not limited to about or at least 1, 2, 3, or 4 weeks or days before and/or after) the initial dose of a target peptide composition.

Exemplary non-limiting doses of the cytokine are in some embodiments about or at least 1-100, 10-80, 20-70, 30-60, 40-50, or 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 Mu/m²/day over about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, or 70 days. The cytokine can in some embodiments be delivered at least or about once every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours. Cytokine treatment can be provided in at least or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 cycles of at least or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more weeks, wherein each cycle has at least or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, or more cytokine doses. Cytokine treatment can in some embodiments be on the same schedule as administration of the target peptide compositions or in some embodiments on a different schedule, which differing schedule can in some embodiments be an overlapping schedule.

In some embodiments, the cytokine is IL-2 and is dosed in an amount about or at least 100,000 to 1,000,000; 200,000-900,000; 300,000-800,000; 450,000-750,000; 600,000-800,000; or 700,000-800,000 (in some embodiments, 720,000) units (IU)/kg administered, e.g., as a bolus, every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 hours for 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or more days, in a cycle, for example.

V. Types of Proliferative Diseases

In some embodiments, the compositions of the presently disclosed subject matter are envisioned to be useful in the treatment of benign and/or malignant proliferative diseases. Excessive proliferation of cells and turnover of cellular matrix contribute significantly to the pathogenesis of several diseases including but not limited to cancer, atherosclerosis, rheumatoid arthritis, psoriasis, idiopathic pulmonary fibrosis, scleroderma and cirrhosis of the liver, ductal hyperplasia, lobular hyperplasia, papillomas, and others.

In some embodiments, the proliferative disease is cancer, including but not limited to breast cancer, colorectal cancer, squamous carcinoma of the lung, sarcoma, renal cell carcinoma, pancreatic carcinomas, squamous tumors of the head and neck, leukemia, brain cancer, liver cancer, prostate cancer, ovarian cancer, and cervical cancer. In some embodiments, the presently disclosed compositions and methods are used to treat colorectal cancer, acute myelogenous leukemia (AML), acute lyphocytic leukemia (ALL), chronic lymphocytic lymphoma (CLL), chronic myelogenous leukemia (CML), breast cancer, renal cancer, pancreatic cancer, and/or ovarian cancer.

In some embodiments, the target peptide compositions of the presently disclosed subject matter can be used to treat colorectal cancer. Colorectal cancer is typically staged in five stages: Stage 0-IV, with several substages. When metastatic (i.e., Stage IV), the colorectal cancer has in some embodiments spread to the lung, bone, liver, and/or brain.

In some embodiments, the cancer is a cancer described herein. For example, the cancer can be a cancer of the bladder (including but not limited to accelerated and metastatic bladder cancer), breast (including but not limited to estrogen receptor positive breast cancer, estrogen receptor negative breast cancer, HER-2 positive breast cancer, HER-2 negative breast cancer, triple negative breast cancer, and inflammatory breast cancer), colon (including but not limited to colorectal cancer), kidney (including but not limited to renal cell carcinoma), liver, lung (including but not limited to small cell lung cancer and non-small cell lung cancer such as but not limited to adenocarcinoma, squamous cell carcinoma, bronchoalveolar carcinoma and large cell carcinoma), genitourinary tract cancer, including but not limited to ovary (such as but not limited to fallopian, endometrial, and peritoneal cancers), cervix, prostate, and testes, lymphatic system, rectum, larynx, pancreas (including but not limited to exocrine pancreatic carcinoma), stomach (including but not limited to gastroesophageal, upper gastric, and lower gastric cancers), gastrointestinal cancer (including but not limited to anal cancer), gall bladder, thyroid, lymphoma (including but not limited to Burkitt's, Hodgkin's, and non-Hodgkin's lymphoma), leukemia (including but not limited to acute myeloid leukemia), Ewing's sarcoma, nasoesophageal cancer, nasopharyngeal cancer, neural and glial cell cancers (including but not limited to glioblastoma multiforme), and head and neck cancers. Exemplary non-limiting cancers also include melanoma, breast cancer (including but not limited to metastatic or locally advanced breast cancer), prostate cancer (including but not limited to hormone refractory prostate cancer), renal cell carcinoma, lung cancer (including but not limited to small cell lung cancer and non-small cell lung cancer (including adenocarcinoma, squamous cell carcinoma, bronchoalveolar carcinoma, and large cell carcinoma), pancreatic cancer, gastric cancer (including but not limited to gastroesophageal, upper gastric, and/or lower gastric cancer), colorectal cancer, squamous cell cancer of the head and neck, ovarian cancer (including but not limited to advanced ovarian cancer, platinum-based agent-resistant, and/or relapsed ovarian cancer), lymphoma (including but not limited to Burkitt's, Hodgkin's, or non-Hodgkin's lymphoma), leukemia (including but not limited to acute myeloid leukemia), and gastrointestinal cancer.

VI. Administration of Vaccine Compositions

VI.A. Routes of Administration

The target peptide compositions of the presently disclosed subject matter can be administered parenterally, systemically, topically, or any combination thereof. By way of example and not limitation, composition injections can be performed by intravenous (i.v.) injection, subcutaneous (s.c.) injection, intradermal (i.d.) injection, intraperitoneal (i.p.) injection, and/or intramuscular (i.m.) injection. One or more such routes can be employed. Parenteral administration can be, for example, by bolus injection or by gradual perfusion over time. Alternatively or in addition, administration can be by the oral route.

In some embodiments, an injection is an intradermal (i.d.) injection. The target peptide compositions are in some embodiments suitable for administration of the peptides by any acceptable route such as but not limited to oral (enteral), nasal, ophthal, and transdermal. In some embodiments, the administration is subcutaneous, and in some embodiments the subcutaneous administration is by an infusion pump.

VI.B. Formulations

Pharmaceutical carriers, diluents, and excipients are generally added to the target peptide compositions or (target peptide compositions kits) that are compatible with the active ingredients and acceptable for pharmaceutical use. Examples of such carriers include but are not limited to water, saline solutions, dextrose, and/or glycerol. Combinations of carriers can also be used.

The vaccine compositions of the presently disclosed subject matter can further incorporate additional substances to stabilize pH and/or to function as adjuvants, wetting agents, and/or emulsifying agents, which can serve to improve the effectiveness of the vaccine.

The target peptide compositions can in some embodiments include one or more adjuvants such as for example: montanide ISA-51 (Seppic Inc., Fairfield, N.J., United States of America); QS-21 (Aquila Biopharmaceuticals, Inc., Framingham, Mass., United States of America); Arlacel A; oeleic acid; tetanus helper peptides (such as but not limited to QYIKANSKFIGITEL (SEQ ID NO: 368) or AQYIKANSKFIGITEL (SEQ ID NO: 205)); GM-CSF; cyclophosamide; bacillus Calmette-Guerin (BCG); Corynbacterium parvum; levamisole, azimezone; isoprinisone; dinitrochlorobenezene (DNCB); keyhole limpet hemocyanin (KLH); Freunds adjuvant (complete and incomplete); mineral gels; aluminum hydroxide (Alum); lysolecithin; pluronic polyols; polyanions; peptides; oil emulsions; nucleic acids (such as but not limited to double-stranded RNAs; dsRNA) dinitrophenol; diphtheria toxin (DT); toll-like receptor (TLR; such as but not limited to TLR3, TLR4, TLR7, TLR8, and/or TLR9) agonists (including but not limited to endotoxins such as lipopolysaccharide (LPS); monophosphoryl lipid A (MPL); and/or polyinosinic-polycytidylic acid (poly-ICLC/HILTONOL®; Oncovir, Inc., Washington, D.C., United States of America); IMO-2055; glucopyranosyl lipid A (GLA); QS-21 (a saponin extracted from the bark of the Quillaja saponaria tree, also known as the soap bark tree or Soapbark); resiquimod (a TLR7/8 agonist); CDX-1401 (a fusion protein consisting of a fully human monoclonal antibody with specificity for the dendritic cell receptor DEC-205 linked to the NY-ESO-1 tumor antigen); Juvaris' Cationic Lipid-DNA Complex; Vaxfectin; and combinations thereof.

In some embodiments, the tetanus peptide can be about or at least 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, (in some embodiments 10-25) natural or non-natural amino acids in length. In some embodiments, the tetanus peptide is a segment or fragment of a tetanus toxoid protein. In some embodiments the tetanus toxoid peptide used herein is at least or about 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to a 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids segment of the wild type tetanus toxoid protein. In some embodiments the tetanus toxoid peptide used herein is at least or about 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99%, or 100% identical to SEQ ID NOs: 205 or 368. In some embodiments, the tetanus peptide binds to one or more MHC Class II molecules. In some embodiments, the tetanus peptide is modified so as to prevent formation of tetanus peptide secondary structures. In some embodiments, a nucleic acid (e.g., DNA or RNA) is provided that encodes a tetanus peptide. In some embodiments, the tetanus peptide belongs to the 830 to 844 amino acid sequence of the tetanus toxin Tc (see Demotz el al., 1989; El Kasmi et al., 2000).

Polyinosinic-Polycytidylic acid (Poly IC) is a double-stranded RNA (dsRNA) that acts as a TLR3 agonist. To increase half-life, it has been stabilized with polylysine and carboxymethylcellulose as poly-ICLC. It has been used to induce interferon in cancer patients, with intravenous doses up to 300 μg/kg. Like poly-IC, poly-ICLC is a TLR3 agonist. TLR3 is expressed in the early endosome of myeloid DC; thus poly-ICLC preferentially activates myeloid dendritic cells, thus favoring a Th1 cytotoxic T cell response. Poly-ICLC activates natural killer (NK) cells, induces cytolytic potential, and induces IFNγ from myeloid DC.

In some embodiments, an adjuvant is provided at about or at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, or 1000 μg per dose or per kg in each dose. In some embodiments, the adjuvant is provided in a dosage of at least or about 0.1, 0.2, 0.3, 0.40, 0.50, 0.60, 0.70, 0.80, 0.90, 0.100, 1.10, 1.20, 1.30, 1.40, 1.50, 1.60, 1.70, 1.80, 1.90, 2.00, 2.10, 2.20, 2.30, 2.40, 2.50, 2.60, 2.70, 2.80, 2.90, 3.00, 3.10, 3.20, 3.30, 3.40, 3.50, 3.60, 3.70, 3.80, 3.90, 4.00, 4.10, 4.20, 4.30, 4.40, 4.50, 4.60, 4.70, 4.80, 4.90, 5.00, 5.10, 5.20, 5.30, 5.40, 5.50, 5.60, 5.70, 5.80, 5.90, 6.00, 6.10, 6.20, 6.30, 6.40, 6.50, 6.60, 6.70, 6.80, 6.90, 7.00, 7.10, 7.20, 7.30, 7.40, 7.50, 7.60, 7.70, 7.80, 7.90, 8.00, 8.10, 8.20, 8.30, 8.40, 8.50, 8.60, 8.70, 8.80, 8.90, 9.00, 9.10, 9.20, 9.30, 9.40, 9.50, 9.60, 9.70, 9.80, 9.90, or 10.00 grams per dose or per kg in each dose. In some embodiments, the adjuvant is given at about or at least 10, 15, 20, 25, 50, 75, 100, 125, 150, 175, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 500, 525, 550, 575, 600, 625, 675, 700, 725, 750, 775, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900 or 2000 endotoxin units (“EU”) per dose.

The target peptide compositions of the presently disclosed subject matter can in some embodiments be provided with an administration of cyclophosamide around the time (such as but not limited to about or at least 1, 2, 3, or 4 weeks or days before or after) of the initial dose of a target peptide composition. Exemplary non-limiting dose of cyclophosamide are in some embodiments about or at least 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 Mg/m²/day over about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 days.

The compositions may comprise the target peptides in the free form and/or in the form of a pharmaceutically acceptable salt. As used herein, “a pharmaceutically acceptable salt” refers to a derivative of a disclosed target peptide wherein the target peptide is modified by making acid or base salts of the agent. For example, acid salts are prepared from the free base (typically wherein the neutral form of the drug has a neutral —NH₂ group) involving reaction with a suitable acid. Suitable acids for preparing acid salts include both organic acids such as but not limited to acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, malic acid, malonic acid, succinic acid, maleic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like, as well as inorganic acids such as but not limited to hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid phosphoric acid, and the like. Conversely, basic salts of acid moieties that can be present on a target peptide are in some embodiments prepared using a pharmaceutically acceptable base such as but not limited to sodium hydroxide, potassium hydroxide, ammonium hydroxide, calcium hydroxide, trimmethylamine, and the like. By way of example and not limitation, the compositions can comprise target peptides as salts of acetic acid (acetates), ammonium, or hydrochloric acid (chlorides).

In some embodiments, a composition can include one or more sugars, sugar alcohols, amino acids such but not limited to glycine, arginine, glutamic acid, and/or others as framework formers. The sugars can be mono-, di-, or trisaccharides. These sugars can be used alone and/or in combination with sugar alcohols. Exemplary sugars include glucose, mannose, galactose, fructose, or sorbose as monosaccharides; sucrose, lactose, maltose, and trehalose as disaccharides; and raffinose as a trisaccharide. A sugar alcohol can be, for example, mannitose. In some embodiments, the composition comprises sucrose, lactose, maltose, trehalose, mannitol, and/or sorbitol. In some embodiments, the composition comprises mannitol.

Furthermore, in some embodiments compositions can include physiological well-tolerated excipients (see Handbook of Pharmaceutical Excipients. 5^(th) ed., edited by Raymond Rowe, Paul Sheskey and Sian Owen, Pharmaceutical Press (2006)) such as antioxidants like ascorbic acid or glutathione; preserving agents such as phenol, m-cresol, methyl- or propylparabene, chlorobutanol, thiomersal/thimerosal, and/or benzalkoniumchloride; stabilizers, framework formers such as sucrose, lactose, maltose, trehalose, mannitose, mannitol, and/or sorbitol; mannitol and/or lactose and solubilizers such as polyethylene glycols (PEG; e.g., PEG 3000, 3350, 4000, or 6000), cyclodextrins (e.g., hydroxypropyl-β-cyclodextrin, sulfobutylethyl-β-cyclodextrin, or γ-cyclodextrin), dextrans, or poloxamers (e.g., poloxamer 407 or poloxamer 188); or TWEEN® 20 or TWEEN® 80. In some embodiments, one or more well-tolerated excipients can be included, optionally selected from the group consisting of antioxidants, framework formers, and stabilizers.

In some embodiments, the pH for intravenous and/or intramuscular administration is selected from pH 2 to pH 12. In some embodiments, the pH for subcutaneous administration is selected from pH 2.7 to pH 9.0 as the rate of in vivo dilution is reduced resulting in more potential for irradiation at the injection site (Strickley, 2004).

VI.C. Dosage

It is understood that a suitable dosage of a target peptide composition vaccine immunogen cam depend upon the age, sex, health, and/or weight of the recipient, the kind of concurrent treatment, if any, the frequency of treatment, and the nature of the effect desired. However, it is understood that dosages can be tailored to the individual subject, as determined by the researcher or clinician. The total dose required for any given treatment will in some embodiments be determined with respect to a standard reference dose based on the experience of the researcher or clinician, such dose being administered either in a single treatment or in a series of doses, the success of which will depend on the production of a desired immunological result (such as but not limited to successful production of a T helper cell and/or CTL-mediated response to the target peptide immunogen composition, which response gives rise to the prevention and/or treatment desired).

Thus, in some embodiments the overall administration schedule is considered in determining the success of a course of treatment and not whether a single dose, given in isolation, would or would not produce the desired immunologically therapeutic result or effect.

Thus, the therapeutically effective amount (i.e., in some embodiments that amount that produces a desired T helper cell and/or CTL-mediated response) can depend on the antigenic composition of the vaccine used, the nature of the disease condition, the severity of the disease condition, the extent of any need to prevent such a condition where it has not already been detected, the manner of administration dictated by the situation requiring such administration, the weight and state of health of the individual receiving such administration, and/or the sound judgment of the clinician or researcher. In some embodiments, the efficacy of administering additional doses and/or of increasing or decreasing the interval can be continually re-evaluated in view of the recipient's immunocompetence (including but not limited to the level of T helper cell and/or CTL activity with respect to tumor-associated or tumor-specific antigens).

The concentration of the T helper or CTL stimulatory target peptides of the presently disclosed subject matter in pharmaceutical formulations can be subject to wide variation, including anywhere from less than 0.01% by weight to as much as 50% or more. Factors such as volume and viscosity of the resulting composition can in some embodiments also be considered. The solvents or diluents used for such compositions can include water, phosphate buffered saline (PBS), and/or saline, or any other possible carriers or excipients.

The immunogens of the presently disclosed subject matter can in some embodiments also be contained in artificially created structures such as liposomes, which structures in some embodiments can contain additional molecules such as but not limited to proteins or polysaccharides, inserted in the outer membranes of said structures and having the effect of targeting the liposomes to particular areas of the body and/or to particular cells within a given organ or tissue. Such targeting molecules can in some embodiments comprise an immunoglobulin. Antibodies can work particularly well for targeting of liposomes and/or other scaffolds to tumor cells.

Single i.d., i.m., s.c., i.p., and/or i.v. doses of in some embodiments about 1 to 50 μg, in some embodiments about 1 to 100 μg, in some embodiments about 1 to 500 μg, in some embodiments about 1 to 1000 μg, in some embodiments about 1 to 50 mg, in some embodiments about 1 to 100 mg, in some embodiments about 1 to 500 mg, or in some embodiments about 1 to 1000 mg of target peptide composition can be given and can depend from the respective compositions of target peptides with respect to total amount for all target peptides in the composition or alternatively for each individual target peptide in the composition. A single dose of a target peptide vaccine composition of the presently disclosed subject matter can in some embodiments have a target peptide amount (e.g., total amount for all target peptides in the composition or alternatively for each individual target peptide in the composition) of about or at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, 600, 625, 650, 675, 700, 725, 750, 775, 800, 825, 850, 875, 900, or 950 μg. In some embodiments, a single dose of a target peptide composition of the presently disclosed subject matter can have a total target peptide amount (e.g., total amount for all target peptides in the composition or alternatively for each individual target peptide in the composition) of about or at least 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, 600, 625, 650, 675, 700, 725, 750, 775, 800, 825, 850, 875, 900, or 950 mg. In some embodiments, the target peptides of a composition of the presently disclosed subject matter are present in equal amounts of about 100 micrograms per dose in combination with an adjuvant peptide present in an amount of about 200 micrograms per dose.

In a single dose of the target peptide composition of the presently disclosed subject matter, the amount of each target peptide in the composition is in some embodiments equal or substantially equal. Alternatively, a ratio of the target peptides present in the least amount relative to the target peptide present in the greatest amount is about or at least 1:1.25, 1:1.5, 1:1.75, 1:2.0, 1:2.25, 1:2.5, 1:2.75, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:20, 1:30; 1:40, 1:50, 1:100, 1:200, 1:500, 1:1000, 1:5000; 1:10,000; or 1:100,000. Alternatively, a ratio of the target peptides present in the least amount relative to the target peptide present in the greatest amount is about or at least 1 or 2 to 25; 1 or 2 to 20; 1 or 2 to 15; 1 or 2 to 10; 1 to 3; 1 to 4; 1 to 5; 1 to 6; 1 to 7; 1 to 10; 2 to 3; 2 to 4; 2 to 5; 2 to 6; 2 to 7; 2 to 10; 3 to 4; 3 to 5; 3 to 6; 3 to 7; 3 to 10; 5 to 10; 10 to 15; 15 to 20:20 to 25; 1 to 40; 1 to 30; 1 to 20; 1 to 15; 10 to 40; 10 to 30; 10 to 20; 10 to 15; 20 to 40; 20 to 30; or 20 to 25; 1 to 100; 25 to 100; 50 to 100; 75 to 100; 25 to 75, 25 to 50, or 50 to 75; 25 to 40; 25 to 50; 30 to 50; 30 to 40; or 30 to 75.

Single dosages can be given to a patient about or at least 1, 2, 3, 4, or 5 times per day. Single dosages can be given to a patient about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19, 20, 21, 22, 23, 24, 36, 48, 60, or 72 hours subsequent to a previous dose.

Single dosages can be given to a patient about or at least 1, 2, 3, 4, 5, 6, or 7 times per week, or every other, third, fourth, or fifth day. Single doses can also be given every week, every other week, or only during 1, 2, or 3 weeks per month. A course of treatment can in some embodiments last about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months.

In some embodiments, the single dosages of the compositions of the presently disclosed subject matter can be provided to a patient in at least two phases: e.g., during an initial phase and then during a subsequent phase. An initial phase can be about or at least 1, 2, 3, 4, 5, or 6 weeks in length. The subsequent phase can last at least or about 1, 2, 3, 4, 5, 6, 7, or 8 times as long as the initial phase. The initial phase can be separated from the subsequent phase by about or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 weeks or months.

The target peptide composition dosage during the subsequent phase can be at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000 times greater than during the initial phase.

The target peptide composition dosage during the subsequent phase can be at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000 times less than during the initial phase.

In some embodiments, the initial phase is about three weeks and the second phase is about 9 weeks. The target peptide compositions can be administered to the patient on or about days 1, 8, 15, 36, 57, and 78.

VI.D. Kits and Storage

In some embodiments, a kit is disclosed comprising (a) a container that contains at least one target peptide composition as described herein, in solution or in lyophilized form; (b) optionally, a second container containing a diluent or reconstituting solution for the lyophilized formulation; and (c) optionally, instructions for (i) use of the solution or (ii) reconstitution and/or use of the lyophilized formulation. The kit may further comprise one or more of (iii) a buffer, (iv) a diluent, (v) a filter, (vi) a needle, or (v) a syringe. In some embodiments, the container is selected from the group consisting of: a bottle, a vial, a syringe, a test tube, or a multi-use container. In some embodiments, the target peptide composition is lyophilized.

The kits can contain exactly, about, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 45, 46, 47, 48, 49, 50, 51, or more target peptide-containing compositions. Each composition in the kit can be administered at the same time and/or at different times.

In some embodiments, the kits can comprise a lyophilized formulation of the presently disclosed compositions and/or vaccines in a suitable container and instructions for its reconstitution and/or use. Suitable containers include, for example, bottles, vials (e.g., dual chamber vials), syringes (such as dual chamber syringes), and test tubes. The container can be formed from a variety of materials such as glass or plastic. In some embodiments, the kit and/or the container contain(s) instructions on or associated therewith that indicate(s) directions for reconstitution and/or use of a lyophilized formulation. For example, the label can indicate that the lyophilized formulation is to be reconstituted to target peptide concentrations as described herein. The label can further indicate that the formulation is useful or intended for subcutaneous administration. Lyophilized and liquid formulations are typically stored at −20° C. to −80° C.

The container holding the target peptide composition(s) can be a multi-use vial, which in some embodiments allows for repeat administrations (e.g., from 2-6 or more administrations) of the reconstituted formulation. The kit can further comprise a second container comprising a suitable diluent (e.g., sodium bicarbonate solution).

In some embodiments, upon mixing of the diluent and the lyophilized formulation, the final peptide concentration in the reconstituted formulation is at least or about 0.15, 0.20, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 1.75, 2.0, 2.25, 2.5, 2.75, 3.0, 3.25, 3.50, 3.75, 4.0, 4.25, 4.5, 4.75, 5.0, 6.0, 7.0, 8.0, 9.0, or 10 mg/mL/target peptide. In some embodiments, upon mixing of the diluent and the lyophilized formulation, the final peptide concentration in the reconstituted formulation is at least or about 0.15, 0.20, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 1.75, 2.0, 2.25, 2.5, 2.75, 3.0, 3.25, 3.50, 3.75, 4.0, 4.25, 4.5, 4.75, 5.0, 6.0, 7.0, 8.0, 9.0, or 10 μg/mL/target peptide.

The kit can further include other materials desirable from a commercial and/or user standpoint, including other buffers, diluents, filters, needles, syringes, and package inserts with or without instructions for use.

The kits can have a single container that contains the formulation of the target peptide compositions with or without other components (e.g., other compounds or compositions of these other compounds) or can have a distinct container for each component.

Additionally, the kits can include a formulation of the presently disclosed target peptide compositions and/or vaccines packaged for use in combination with the co-administration of a second compound such as an adjuvant including but not limited to imiquimod, a chemotherapeutic agent, a natural product, a hormone or antagonist, an anti-angiogenesis agent or inhibitor, an apoptosis-inducing agent or a chelator) or a composition thereof. One or more of the components of the kit can be pre-complexed or one or more components can be in a separate distinct container prior to administration to a patient. One or more of the components of the kit can be provided in one or more liquid solutions. In some embodiments, the liquid solution is an aqueous solution. In some embodiments, the liquid solution is a sterile aqueous solution. One or more of the components of the kit can also be provided as solids, which in some embodiments can be converted into liquids by addition of suitable solvents, which in some embodiments can be provided in another distinct container.

The container of a therapeutic kit can be a vial, a test tube, a flask, a bottle, a syringe, or any other structure suitable for enclosing a solid or liquid. Typically, when there is more than one component, the kit contains a second vial or other container that allows for separate dosing. The kit can also contain another container for a pharmaceutically acceptable liquid. In some embodiments, a therapeutic kit contains an apparatus (e.g., one or more needles, syringes, eye droppers, pipette, etc.), which enables administration of the agents of the disclosure that are components of the kit.

VI.E. Markers for Efficacy

When administered to a patient, the vaccine compositions of the presently disclosed subject matter are in some embodiments envisioned to have certain physiological effects including but not limited to the induction of a T cell mediated immune response.

VI.E.1. Immunohistochemistry, Immunofluorescence. Western Blots, and Flow Cytometry

Validation and testing of antibodies for characterization of cellular and molecular features of lymphoid neogenesis has been performed. Commercially available antibodies for use in immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and/or western blotting (WB) can be used. In some embodiments, such techniques can be employed to assay patient samples including but not limited to formalin-fixed, paraffin-embedded tissue samples for the presence or absence of and/or for a level of expression of one or more of CD1a, S100, CD83, DC-LAMP, CD3, CD4, CD8, CD20, CD45, CD79a, PNAd, TNFα, LIGHT, CCL19, CCL21, CXCL12, TLR4, TLR7, FoxP3, PD-1, and Ki67 gene products. In some embodiments, flow cytometry is used to determine an expression level for one or more of CD3, CD4, CD8, CD13, CD14, CD16, CD19, CD45RA, CD45RO, CD56, CD62L, CD27, CD28, CCR7, FoxP3 (intracellular), and MHC-peptide tetramers for class I MHC associated (phospho)-peptides. In some embodiments, a positive control is employed, which in some embodiments can comprise a tissue sample comprising normal human peripheral blood lymphocytes (PBL), PBL activated with CD3/CD28 beads (activated PBL), human lymph node tissue from non-colorectal cancer patients (LN), and/or inflamed human tissue from a surgical specimen of Crohn's disease (Crohn's), although any other positive control cell and/or tissue can be employed.

VI.E.2. ELISpot Assay

In some embodiments, vaccination site infiltrating lymphocytes and lymphocytes from the sentinel immunized node (SIN) and vaccine site can be evaluated by ELISpot. ELISpot permits the direct counting of T cells reacting to antigen by production of IFNγ. Peripheral blood lymphocytes can be evaluated by ELISpot assay for the number of peptide-reactive T cells. Vaccine site infiltrating lymphocytes and SIN lymphocytes can be compared to those in peripheral blood. It is envisioned that positive results of the ELISpot assay correlates with increased patient progression free survival. Progression free survival is defined as the time from start of treatment until death from any cause or date of last follow up.

VI.E.3. Tetramer Assay

Peripheral blood lymphocytes and lymphocytes from the SIN and vaccine site can be evaluated by flow cytometry after incubation with MHC-peptide tetramers for the number of peptide-reactive T cells.

VI.E.4. Proliferation Assay and Cytokine Analysis

Peripheral blood mononuclear cells (PBMC), vaccine-site inflammatory cells, and/or lymphocytes from the SIN isolated from subjects can be evaluated for CD4⁺ T cell reactivity to, e.g., tetanus helper peptide mixture, using a ³H-thymidine uptake assay. Additionally, Th1 (IL-2, IFNγ, TNFα), Th2 (IL-4, IL-5, IL-10), Th17 (IL-17, and IL23), and T-reg (TGF-β) cytokines in media from 48 hours in that proliferation assay can be used to determine if the microenvironment supports generation of Th1, Th2, Th17, and/or T-reg responses. In some embodiments, one or both of the following peptides are used as negative controls: a tetanus peptide and the PADRE peptide (AK(X)VAAWTLKAA; SEQ ID NO: 367.

VI.E.5. Evaluation of Tumors

In some embodiments, tumor tissue collected prior to treatment or at the time of progression can be evaluated by routine histology and immunohistochemistry. Alternatively or in addition, in vitro evaluations of tumor tissue and tumor infiltrating lymphocytes can be performed.

VI.E.6. Studies of Homing Receptor Expression

Patient samples can be studied for T cell homing receptors induced by vaccination with the compositions of the presently disclosed subject matter. These include but are not limited to integrins (including but not limited to αEβ7, α1β1, α4β1), chemokine receptors (including but not limited to CXCR3), and selectin ligands (including but not limited to CLA and PSL) on lymphocytes, and their ligands in the vaccine sites and SIN. In some embodiments, these can be assayed by immunohistochemistry, flow cytometry, and/or any other appropriate technique(s).

VI.E.7. Studies of Gene and Protein Expression

Differences in gene expression and/or differences in protein expression profiles can be determined by high-throughput screening assays (e.g., nucleic acid chips, protein arrays, etc.) of samples isolated from vaccine sites and/or SIN.

VII. Antibodies and Antibody-Like Molecules

Antibodies and antibody-like molecules (including but not limited to T cell receptors) specific for target peptides and/or target peptide/MHC complexes are in some embodiments useful for analyzing biological samples. In some embodiments, an analysis can comprise determining the pathological nature of tumor margins.

Antibodies and antibody-like molecules can also be used as therapeutics. In some embodiments, such molecules can be used as therapeutics that target cells, including but not limited to tumor cells, which display target peptides on their surfaces. In some embodiments, antibodies and antibody-like molecules bind to phosphorylated target peptides and/or target peptide-MHC complex specifically and do not substantially cross react with the corresponding non-phosphorylated native peptides.

As used herein, the terms “antibody”, “antibody peptide(s)”, and “antibody-like molecule(s)” refer to an intact antibody, a binding fragment thereof (i.e., a fragment of an antibody that comprises a paratope), or a polypeptide that can specifically recognize an antigen or epitope and bind to the same in a fashion that mimics antibody binding. In some embodiments, antibodies, antibody peptides, and antibody-like molecules compete with intact antibodies for specific binding to an antigen or epitope.

In some embodiments, antibody fragments can be produced by recombinant DNA techniques and/or by enzymatic and/or chemical cleavage of intact antibodies. Antibody fragments thus include but are not limited to Fab fragments, Fab′ fragments, F(ab′)₂ fragments, Fv, and single-chain antibodies including but not limited to single-chain fragment variable (scFv) antibodies. An antibody is said to be “monospecific” if each of its paratopes is identical and/or binds to the same epitope. Similarly, “bispecific” or “bifunctional” antibodies comprise paratopes that bind to different antigens and/or epitopes. In some embodiments, an antibody substantially inhibits adhesion of a receptor to a counterreceptor when an excess of antibody reduces the quantity of receptor bound to counterreceptor by at least about 20%, 40%, 60%, 80%, 85%, 90%, 95%, or more as measured by, for example, an in vitro competitive binding assay.

The term “MHC” as used herein refers to the Major Histocompability Complex, which is defined as a set of gene loci specifying major histocompatibility antigens. The term “HLA” as used herein will be understood to refer to Human Leukocyte Antigens, which is defined as the histocompatibility antigens found in humans. As used herein, “HLA” is the human form of “MHC”. IN murine species, the MHC is referred to as the “H-2” complex.

The terms “MHC light chain” and “MHC heavy chain” as used herein refer to particular portions of a MHC molecule. Structurally, class I molecules are heterodimers comprised of two noncovalently bound polypeptide chains, a larger “heavy” chain (α) and a smaller “light” chain (β2-microglobulin or β2m). The polymorphic, polygenic heavy chain (45 kDa), encoded within the MHC on chromosome human 6 is subdivided into three extracellular domains (designated 1, 2, and 3), one intracellular domain, and one transmembrane domain. The two outermost extracellular domains, 1 and 2, together form the groove that binds to antigenic peptides and/or other epitopes. Thus, interaction with the TCR occurs at this region of the protein. Domain 3 of the molecule contains the recognition site for the CD8 protein on the CTL. This interaction serves to stabilize the contact between the T cell and an antigen-presenting cell (APC). The invariant light chain (12 kDa), encoded on human chromosome 15, consists of a single, extracellular polypeptide. The terms “MHC light chain”, “β2-microglobulin”, and “β2m” are used interchangeably herein.

The term “epitope” includes any protein determinant capable of specific binding to an antibody, antibody peptide, and/or antibody-like molecule (including but not limited to a T cell receptor) as defined herein. Epitopic determinants typically consist of chemically active surface groups of molecules such as amino acids or sugar side chains and generally have specific three dimensional structural characteristics as well as specific charge characteristics. An antibody or antibody-like molecule is said to “specifically” bind an antigen when the dissociation constant (K_(d)) is in some embodiments less than about 1 μM, in some embodiments less that about 100 nM, and in some embodiments less than about 10 nM. Interactions between antibodies and antibody-like molecules and an epitope can also be characterized by an affinity constant (K_(a)). In some embodiments, a K_(a) of less than about 10⁷/M is considered “high affinity”.

The term “antibody” is used in the broadest sense, and covers monoclonal antibodies (including full length monoclonal antibodies), polyclonal antibodies, multispecific antibodies (e.g., bispecific and/or trispecific antibodies), and antibody fragments (including but not limited to Fab, F(ab′)₂ and Fv fragments) as well as antibody-like molecules provided that they exhibit the desired biological activity (e.g., antigen binding). Antibodies (Abs) and immunoglobulins (Igs) are glycoproteins that in some embodiments have the same structural characteristics. The term is also meant to encompass “antibody like molecules” and other members of the immunoglobulin superfamily including, but not limited to T cell receptors, MHC molecules, and other polypeptides that contain one or more antigen-binding regions and/or variable regions including, but not limited to complementary determining regions (CDRs) that specifically bind the target peptides disclosed herein.

In some embodiments, antibodies and antibody-like molecules bind to the target peptides disclosed herein but do not substantially and/or specifically crossreact with the same peptide in a modified form. See e.g., U.S. Patent Application Publication No. 2009/0226474, which is incorporated by reference.

The presently disclosed subject matter includes in some embodiments antibodies that recognize target peptides associated with a tumorigenic or disease state, wherein the peptides are displayed in the context of HLA molecules. These antibodies can mimic the specificity of a T cell receptor (TCR) but can have higher binding affinities such that the molecules can be employed as therapeutic, diagnostic, and/or research reagents. Methods of producing a T cell receptor mimic of the presently disclosed subject matter in some embodiments comprise identifying a target peptide of interest, generating and isolating CD8⁺ T cells specific for the target peptide, and cloning gene sequences that encode the target peptide-specific TCR.

In some embodiments an immunogen comprising at least one target peptide/MHC complex is formed. An effective amount of the immunogen is in some embodiments administered to a host to elicit an immune response in the host, and serum collected from the host can assayed to determine if antibodies that recognize a three-dimensional presentation of the target peptide in the binding groove of the MHC molecule have been produced. The desired antibodies can in some embodiments differentiate the target peptide/MHC complex from the MHC molecule alone, the target peptide alone, and/or a complex of MHC and an irrelevant peptide (in some embodiments, a peptide having the same amino acid composition as a target peptide but wherein the amino acids are in a different order that in the target peptide). Finally, in some embodiments the desired antibodies can be isolated.

The term “antibody” also encompasses soluble T cell receptor (TCR) cytoplasmic domains that are stable at low concentrations and which can recognize MHC-peptide complexes. See e.g., U.S. Patent Application Publication No. 2002/0119149, which is incorporated by reference. Such soluble TCRs can in some embodiments be conjugated to immunostimulatory peptides and/or proteins, and/or moieties such as but not limited to CD3 agonists (e.g., anti-CD3 antibodies). The CD3 antigen is present on mature human T cells, thymocytes, and a subset of natural killer cells. It is associated with the TCR and is involved in signal transduction of the TCR. Antibodies specific for the human CD3 antigen are well known. One such antibody is the murine monoclonal antibody OKT3 which was the first monoclonal antibody approved by the FDA. OKT3 is reported to be a potent T cell mitogen (Van Wauve, 1980; U.S. Pat. No. 4,361,539) and a potent T cell killer (Wong, 1990). Other antibodies specific for the CD3 antigen have also been reported (see PCT International Patent Application Publication No. WO 2004/106380; U.S. Patent Application Publication No. 2004/0202657; U.S. Pat. Nos. 6,750,325; 6,706,265; United Kingdom Patent GB 2249310A; Clark et al., 1989; U.S. Pat. No. 5,968,509; and U.S. Patent Application Publication No. 2009/0117102). Immune mobilizing mTCR Against Cancer (ImmTAC; Immunocore Limited, Milton Park, Abington, Oxon, United Kingdom) are bifunctional proteins that combine high-affinity monoclonal T cell receptor (mTCR) targeting with a therapeutic mechanism of action (i.e., an anti-CD3 scFv).

Native antibodies and immunoglobulins are generally heterotetrameric glycoproteins of about 150,000 daltons (Da) composed of two identical light (L) chains and two identical heavy (H) chains. Each light chain is linked to a heavy chain by a covalent disulfide bond. Disulfide bonds also link the heavy chains of intact antibodies, although the number of disulfide bonds between the heavy chains of different immunoglobulin isotypes can vary. Each heavy and light chain also has regularly spaced intrachain disulfide bridges. Each heavy chain has at one end a variable domain (V_(H)) followed by a number of constant domains. Each light chain has a variable domain at one end (V_(L)) and a constant domain at its other end. The constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain. Particular amino acid residues are believed to form an interface between the light and heavy chain variable domains (Clothia et al., 1985; Novotny & Haber, 1985).

An “isolated” antibody is one which has been identified and/or separated and/or recovered from a component of the environment in which it was produced or otherwise present. Contaminant components of its production environment are materials that in some embodiments interfere with diagnostic and/or therapeutic uses for the antibody, and in some embodiments can include enzymes, hormones, and other proteinaceous or nonproteinaceous solutes. In some embodiments, an antibody can be purified as measurable by one or more of the following methods: 1) to greater than 50%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% by weight of antibody as determined by the Lowry method; 2) to a degree sufficient to obtain at least 10 or 15 residues of N-terminal or internal amino acid sequence by use of a spinning cup sequenator; or 3) to homogeneity by SDS-PAGE under reducing or non-reducing conditions using Coomassie blue or, in some embodiments, silver stain. Isolated antibodies include an antibody in situ within recombinant cells since at least one component of the antibody's natural environment will not be present. Ordinarily, however, isolated antibodies will be prepared by a method that comprises at least one purification step.

The terms “antibody mutant” and “antibody variant” refer to antibodies that relative to a reference antibody comprise one or more amino acid sequence differences, wherein one or more of the amino acid residues have been modified such as but not limited to substitution and/or deletion. Such mutants and/or variants comprise in some embodiments less than 100%, 99%, 98%, 97%, 96%, 95%, 90%, 85%, 80%, or 75% sequence identity and/or similarity to the amino acid sequence of either the heavy or light chain variable domain amino acid sequence of the reference antibody.

The term “variable” in the context of variable domain of antibodies, refers to the fact that certain portions of the variable domains differ extensively in sequence among antibodies and are used in the binding and specificity of each particular antibody for its particular antigen. However, sequence variability is generally not evenly distributed throughout the variable domains of antibodies. Typically, sequence variability is concentrated in three segments called complementarity determining regions (CDRs; also known as hypervariable regions) both in the light chain and heavy chain variable domains.

There are at least two techniques for determining CDRs: (1) an approach based on cross-species sequence variability (i.e., Kabat et al., 1991); and (2) an approach based on crystallographic studies of antigen-antibody complexes (Chothia et al., 1989). The more highly conserved portions of variable domains are called the framework (FR). The variable domains of native heavy and light chains each comprise four FR regions, largely adopting a β-sheet configuration, connected by three CDRs, which form loops connecting, and in some cases forming part of, the β-sheet structure. The CDRs in each chain are held together in close proximity by the FR regions and, with the CDRs from the other chain, contribute to the formation of the antigen binding site of antibodies (see Kabat et al., 1991) The constant domains are generally not involved directly in binding between antibody and antigen, but exhibit various effector functions such as but not limited to participation of the antibody in antibody-dependent cellular toxicity.

The term “antibody fragment” refers to a portion of a full-length antibody, generally the antigen binding or variable region. Examples of antibody fragments include Fab, Fab′, F(ab′)₂, and Fv fragments. Papain digestion of antibodies produces two identical antigen binding fragments, called the Fab fragment, each with a single antigen binding site, and a residual “Fc” fragment, so-called for its ability to crystallize readily. Pepsin treatment yields an F(ab′)₂ fragment that has two antigen binding fragments which are capable of cross-linking antigen, and a residual other fragment (which is termed pFc′). As used herein, the phrase “functional fragment” with respect to antibodies refers in some embodiments to a fragment that contains at least one antigen-binding domain (referred to as a “paratope”), and thus includes, but is not limited to Fv, F(ab) and F(ab′)₂ fragments.

An “Fv” fragment is the minimum antibody fragment which contains a complete antigen recognition and binding site. This region consists of a heterodimer of one heavy and one light chain variable domain in a tight, non-covalent or covalent association (V_(H)-V_(L) dimer). It is in this configuration that the three CDRs of each variable domain interact to define an antigen binding site (paratope) on the surface of the V_(H)-V_(L) dimer. Collectively, the six CDRs confer antigen binding specificity to the antibody. However, in some embodiments even a single variable domain (or half of an Fv comprising only three CDRs specific for an antigen) has the ability to recognize and bind antigen, although at a lower affinity than the entire binding site.

The Fab or F(ab) fragment also contains the constant domain of the light chain and the first constant domain (CH1) of the heavy chain. Fab′ fragments differ from Fab fragments by the addition of a few residues at the carboxyl terminus of the heavy chain CH1 domain including one or more cysteines from the antibody hinge region. Fab′-SH is the designation herein for Fab′ in which the cysteine residue(s) of the constant domains have a free thiol group. F(ab′) fragments are produced by cleavage of the disulfide bond at the hinge cysteines of the F(ab′)₂ pepsin digestion product. Additional chemical couplings of antibody fragments are known to those of ordinary skill in the art.

The light chains of antibodies (immunoglobulin) from any vertebrate species can be assigned to one of two clearly distinct types, called kappa (κ) and lambda (λ), based on the amino sequences of the corresponding constant domain.

Depending on the amino acid sequences of the constant domain of their heavy chains, immunoglobulins can be assigned to different classes. There are at least five (5) major classes of immunoglobulins: IgA, IgD, IgE, IgG and IgM, and several of these may be further divided into subclasses or isotypes (e.g., IgG₁, IgG₂, IgG₃, IgG₄, IgA₁, IgA₂, etc.). The heavy chains constant domains that correspond to the different classes of immunoglobulins are called alpha (α), delta (Δ), epsilon (ε), gamma (γ), and mu (μ), respectively. The subunit structures and three-dimensional configurations of different classes of immunoglobulins are well known.

The term “monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that can be present in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to conventional (polyclonal) antibody preparations, which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen. In additional to their specificity, monoclonal antibodies can be advantageous in that they are typically synthesized from hybridomas and thus can be isolated in a form that is uncontaminated by other immunoglobulins. Methods for generating hybridomas are known in the art. The modifier “monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method. By way of example and not limitation, monoclonal antibodies to be used in accordance with the presently disclosed subject matter can be made by the hybridoma method first described by Kohler & Milstein, 1975, or can be made by recombinant methods (see e.g., U.S. Pat. No. 4,816,567; Harlow & Lane, 1988). In some embodiments, the monoclonal antibodies for use with the presently disclosed subject matter can be isolated from phage antibody libraries using the techniques described in Clackson et al., 1991 and/or Marks et al., 1991.

Utilization of the monoclonal antibodies of the presently disclosed subject matter can in some embodiments comprise administering one or more monoclonal antibodies to a subject, such as but not limited to a human subject. However, when the monoclonal antibodies are produced in a non-human animal, such as a rodent, administration of such antibodies to a human patient can elicit an immune response, wherein the immune response is directed towards the administered antibodies themselves. Such reactions can limit the duration and effectiveness of such a therapy. In order to overcome such a problem, the monoclonal antibodies of the presently disclosed subject matter can in some embodiments be “humanized”, that is, the antibodies are engineered such that antigenic portions thereof are removed and like portions of a human antibody are substituted therefor, while the antibodies' affinity for specific peptide/MHC complexes is retained. This engineering can involve a few amino acids, or can include the entire framework regions of the antibody, leaving only the complementarity determining regions of the parent antibody intact. Several methods of humanizing antibodies are known in the art and are disclosed in U.S. Pat. Nos. 6,180,370; 6,054,927; 5,869,619; 5,861,155; 5,712,120; and 4,816,567, the entire disclosure of each of which is hereby expressly incorporated herein by reference in its entirety.

Humanized forms of antibodies are thus chimeric immunoglobulins, immunoglobulin chains, or fragments thereof (such as Fv, Fab, Fab′, F(ab′)₂ or other antigen-binding subsequences of antibodies) that are principally comprised of the sequence of a human immunoglobulin, but that contain at least some subsequences derived from a non-human immunoglobulin. Humanization can be performed following the method of Winter and co-workers by substituting rodent CDRs or CDR sequences for the corresponding sequences of a human antibody (Jones et al., 1986; Riechmann et al., 1988; Verhoeyen et al., 1988; see also U.S. Pat. No. 5,225,539). In some embodiments, F_(v) framework residues of a human immunoglobulin are replaced with corresponding non-human residues from an antibody of interest. Humanized antibodies can also comprise residues which are found neither in the recipient antibody nor in the imported CDR or framework sequences. In general, the humanized antibody comprises substantially all of at least one, and typically two, variable domains, in which all or substantially all of the CDR regions correspond to those of a non-human immunoglobulin and all or substantially all of the framework regions are those of a human immunoglobulin consensus sequence. The humanized antibody optimally can also comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin (Jones et al., 1986; Riechmann et al., 1988; and Presta, 1992).

Exemplary publications relating to the generation and/or use of humanized antibodies include Sandborn et al., 2001; Mihara et al., 2001; Yenari et al., 2001; Morales et al., 2000; Richards et al., 1999; Yenari et al., 1998; and Shinkura et al., 1998; each of which is expressly incorporated by reference herein in its entirety. For example, a treatment protocol that can be utilized in such a method includes a single dose, generally administered intravenously, of 10-20 mg of humanized mAb per kg (see e.g., Sandborn et al., 2001). In some cases, alternative dosing patterns can be appropriate, such as the use of three infusions, administered once every two weeks, of 800-1600 mg or even higher amounts of humanized mAb (see e.g., Richards et al., 1999). However, it is to be understood that the presently disclosed subject matter is not limited to the treatment protocols described herein, and further that other treatment protocols that are known to one of ordinary skill in the art can be employed in the methods of the presently disclosed subject matter.

In some embodiments, the presently disclosed subject matter further relates to fully human monoclonal antibodies against specific target peptide/MHC complexes. Fully human antibodies essentially relate to antibody molecules in which the entire sequence of both the light chain and the heavy chain, including the CDRs, arise from human genes. Such antibodies are referred to herein as “human antibodies” or “fully human antibodies”.

Human monoclonal antibodies can be prepared by the trioma technique (see U.S. Pat. No. 4,714,681; PCT International Patent Application Publication No. WO 1999/047929); the human B-cell hybridoma technique (see Kozbor et al., 1983), and/or the EBV hybridoma technique (see Cole et al., 1985). In some embodiments, human monoclonal antibodies can be utilized in the practice of the presently disclosed subject matter and can be produced by using human hybridomas (see Cote et al., 1983) or by transforming human B-cells with Epstein Barr Virus in vitro (see Cole et al., 1985). In addition, human antibodies can also be produced using additional techniques, such as but not limited to phage display libraries (Hoogenboom et al., 1991; Marks et al., 1991). Similarly, human antibodies can be made by introducing human immunoglobulin loci into transgenic animals, e.g., mice in which the endogenous immunoglobulin genes have been partially or completely inactivated. Upon challenge, human antibody production is observed, which closely resembles that seen in humans in all respects, including gene rearrangement, assembly, and antibody repertoire. This approach is described, for example, in U.S. Pat. Nos. 5,545,807; 5,545,806; 5,569,825; 5,625,126; 5,633,425; 5,661,016, and in Marks et al., 1992; Lonberg et al., 1994; Fishwild et al., 1996; Neuberger, 1996; and Lonberg & Huszar, 1995.

Human antibodies can additionally be produced using transgenic non-human animals that have been modified to produce fully human antibodies in addition to or rather than the non-human animal's endogenous antibodies in response to challenge by an antigen. See PCT International Patent Application Publication No. WO 1994/02602. In some embodiments, endogenous genes encoding the heavy and light immunoglobulin chains present in the non-human animal have been deleted or otherwise inactivated, and nucleic acids encoding human heavy and light chain immunoglobulins have been inserted into the host's genome. The human genes are incorporated, for example, using yeast artificial chromosomes containing the requisite human DNA segments. An animal that provides all the desired modifications is then obtained as progeny by crossbreeding intermediate transgenic animals containing fewer than the full complement of the modifications.

One embodiment of such a non-human animal is a mouse termed the XENOMOUSE™, which is described in PCT International Patent Application Publication Nos. WO 1996/33735 and WO 1996/34096. The XENOMOUSE™ produces B cells that secrete fully human immunoglobulins. The antibodies can be obtained directly from the animal after immunization with an immunogen of interest, as, for example, a preparation of polyclonal antibodies, or alternatively from immortalized B cells derived from an immunized animal, such as hybridomas producing monoclonal antibodies. Additionally, the genes encoding the immunoglobulins with human variable regions can be recovered and expressed to obtain the antibodies directly and/or can be further modified to obtain analogs of antibodies such as, for example, single chain Fv molecules.

An example of a method for producing a non-human animal such as but not limited to a mouse that lacks expression of an endogenous immunoglobulin heavy chain is disclosed in U.S. Pat. No. 5,939,598, incorporated herein by reference. Such a non-human animal can be obtained by a method that comprises deleting the J segment genes from at least one endogenous heavy chain locus in an embryonic stem cell, thereby preventing rearrangement of the locus and formation of an RNA encoding a rearranged immunoglobulin heavy chain locus. In some embodiments, the deletion can be effected by a targeting vector that contains a selectable marker, Thereafter, a transgenic animal (e.g., a mouse) having somatic and germ cells containing the gene encoding the selectable marker can be produced from the embryonic stem cell. The transgenic animal would be expected to be unable to rearrange its endogenous immunoglobulin heavy chain locus, and thus would be expected to be unable to produce endogenous immunoglobulins.

A method for producing an antibody of interest, such as a human antibody, is also disclosed in U.S. Pat. No. 5,916,771, incorporated herein by reference. It includes introducing a first expression vector that contains a nucleotide sequence encoding a heavy chain into one mammalian host cell in culture, introducing a second expression vector containing a nucleotide sequence encoding a light chain into another mammalian host cell, and fusing the two cells to form a hybrid cell. The hybrid cell can express thus an antibody made up of a heavy chain and a light chain encoded by the first and second expression vectors.

Target peptides disclosed herein are in some embodiments expressed on a variety of cancer cell types. Thus, in some embodiments antibodies and antibody-like molecules can be used in treating, diagnosing, vaccinating, preventing, retarding, and attenuating a cancer such as but not limited to melanoma, ovarian cancer, breast cancer, colorectal cancer, squamous carcinoma of the lung, sarcoma, renal cell carcinoma, pancreatic carcinomas, squamous tumors of the head and neck, leukemia, brain cancer, liver cancer, prostate cancer, ovarian cancer, and cervical cancer.

Antibodies generated with specificity for a target peptide as disclosed herein can be used to detect the corresponding target peptides in a biological sample. The biological sample is in some embodiments isolated from an individual who is suspected of having cancer, and thus detection could serve to diagnose the cancer. Alternatively, the biological sample could be isolated from an individual known to have cancer, and detection of a target peptide therein can serve as an indicator of disease prognosis, cancer characterization, treatment efficacy, disease progression, or any combination thereof. Immunoassays that can be employed for these purposes are known in the art and include, but are not limited to, immunohistochemistry, flow cytometry, radioimmunoassay, western blotting, and ELISA. Biological samples suitable for such testing include, but are not limited to, cells, tissue biopsy specimens, whole blood, plasma, serum, sputum, cerebrospinal fluid, pleural fluid, and urine.

Antigens recognized by T cells, whether helper T lymphocytes or CTL, are not recognized as intact proteins, but rather as small peptides that associate with class I or class II MHC proteins on the surface of cells. During the course of a naturally occurring immune response, antigens that are recognized in association with class II MHC molecules on antigen presenting cells (APCs) are acquired from outside the cell, internalized, and processed into small peptides that associate with the class II MHC molecules.

Antigens that give rise to proteins that are recognized in association with class I MHC molecules are generally proteins that are produced within the cells, and these antigens are processed and associate with class I MHC molecules. It is now understood that the peptides that associate with given class I or class II MHC molecules are characterized as having a common binding motif, and the binding motifs for a large number of different class I and II MHC molecules have been determined. Synthetic peptides can also be synthesized that correspond to the amino acid sequence of a given antigen and that contain a binding motif for a given class I or II MHC molecule. These peptides can then be added to appropriate APCs, and the APCs can be used to stimulate a T helper cell or CTL response either in vitro or in vivo. The binding motifs, methods for synthesizing the peptides, and methods for stimulating a T helper cell or CTL response are all known and readily available to one of ordinary skill in the art.

Kits can be prepared to assist in diagnosis, monitoring, and/or prognosis of diseases. In some embodiments, the kits facilitate the detection and/or measurement of cancer-specific phosphopeptides and/or phosphoproteins. Such kits can contain, in a single or divided container, a molecule comprising an antigen-binding region. In some embodiments, such molecules are antibodies or antibody-like molecules. Additional components that can be included in the kit include one or more of solid supports, detection reagents, secondary antibodies, instructions for use, vessels for running assays, gels, control samples, and the like. In some embodiments, an antibody or antibody-like molecules can optionally be directly or indirectly labeled.

Alternatively, the antibody or antibody-like molecules specific for phosphopeptides and/or phosphopeptide/MHC complexes can be conjugated to therapeutic agents. Exemplary therapeutic agents include, but are not limited to the following:

Alkylating Agents. Alkylating agents are drugs that directly interact with genomic DNA to prevent cells from proliferating. This category of chemotherapeutic drugs represents agents that affect all phases of the cell cycle (i.e., they are not cell cycle phase-specific). Alkylating agents include, but are not limited to nitrogen mustards, ethylenimenes, methylmelamines, alkyl sulfonates, nitrosoureas, and triazines. Particularly exemplary alkylating agents include but are not limited to busulfan, chlorambucil, cisplatin, cyclophosphamide (cytoxan), dacarbazine, ifosfamide, mechlorethamine (mustargen), and melphalan.

Antimetabolites. Antimetabolites disrupt DNA and RNA synthesis. Unlike alkylating agents, they specifically influence the cell cycle during S phase. Antimetabolites can be differentiated into various categories, such as folic acid analogs, pyrimidine analogs, purine analogs, and related inhibitory compounds. Antimetabolites include but are not limited to 5-fluorouracil (5-FU), cytarabine (Ara-C), fludarabine, gemcitabine, and methotrexate.

Natural Products. Natural products generally refer to compounds originally isolated from a natural source and identified as having a desirable pharmacological activity. Such compounds, including analogs and derivatives thereof, can be isolated from a natural source, chemically synthesized, and/or recombinantly produced by any technique known to those of skill in the art. Natural products include such categories as mitotic inhibitors, antitumor antibiotics, enzymes, and biological response modifiers.

Mitotic inhibitors include plant alkaloids and other natural agents that can in some embodiments inhibit protein synthesis required for cell division and in some embodiments inhibit mitosis. They typically operate during a specific phase of the cell cycle. Mitotic inhibitors include, for example, docetaxel, etoposide (VP16), teniposide, paclitaxel, taxol, vinblastine, vincristine, and vinorelbine, among others.

Taxoids are a class of related compounds isolated from the bark of the ash tree, Taxus brevifolia. Taxoids include but are not limited to compounds such as docetaxel and paclitaxel. Paclitaxel binds to tubulin (at a site distinct from that used by the vinca alkaloids) and promotes the assembly of microtubules.

Vinca alkaloids are a type of plant alkaloid identified to have pharmaceutical activity. Exemplary vinca alkaloids include vinblastine (VLB) and vincristine.

Antibiotics. Certain antibiotics have both antimicrobial and/or cytotoxic activity. These drugs also interfere with DNA by chemically inhibiting enzymes and mitosis or altering cellular membranes. These agents are typically not cell cycle phase-specific. Examples of cytotoxic antibiotics include but are not limited to bleomycin, dactinomycin, daunorubicin, doxorubicin (Adriamycin), plicamycin (mithramycin), and idarubicin.

Miscellaneous Agents. Miscellaneous cytotoxic agents that do not fall into the previous categories include but are not limited to platinum coordination complexes, anthracenediones, substituted ureas, methyl hydrazine derivatives, amsacrine, L-asparaginase, and tretinoin. Platinum coordination complexes include such compounds as carboplatin and cisplatin (cis-DDP). An exemplary anthracenedione is mitoxantrone. An exemplary substituted urea is hydroxyurea. An exemplary methyl hydrazine derivative is procarbazine (N-methylhydrazine, MIH). These examples are non-limiting and it is contemplated that any known cytotoxic, cytostatic, and/or cytocidal agent can be attached to a targeting peptide of the presently disclosed subject matter and administered to a targeted organ, tissue, and/or cell type.

Chemotherapeutic (cytotoxic) agents including, but are not limited to, 5-fluorouracil, bleomycin, busulfan, camptothecin, carboplatin, chlorambucil, cisplatin (CDDP), cyclophosphamide, dactinomycin, daunorubicin, doxorubicin, estrogen receptor binding agents, etoposide (VP16), farnesyl-protein transferase inhibitors, gemcitabine, ifosfamide, mechlorethamine, melphalan, mitomycin, navelbine, nitrosurea, plicomycin, procarbazine, raioxifene, tamoxifen, taxol, temazolomide (an aqueous form of DTIC), transplatinum, vinblastine, methotrexate, vincristine, and any analogs and/or derivatives or variants of the foregoing. Most chemotherapeutic agents fall into the categories of alkylating agents, antimetabolites, antitumor antibiotics, corticosteroid hormones, mitotic inhibitors, and nitrosoureas, hormone agents, miscellaneous agents, and any analog, derivative, or variant thereof.

The peptides identified and tested thus far in peptide-based vaccine approaches have generally fallen into one of three categories: 1) mutated on individual tumors, and thus not displayed on a broad cross-section of tumors from different patients; 2) derived from unmutated tissue-specific proteins, and thus compromised by mechanisms of self-tolerance; and 3) expressed in subsets of cancer cells and normal testes.

Antigens linked to transformation or oncogenic processes are of primary interest for immunotherapeutic development based on the hypothesis that tumor escape through mutation of these proteins could be more difficult without compromising tumor growth or metastatic potential

The target peptides of the presently disclosed subject matter are in some embodiments unique in that the identified target peptides are modified by intracellular modification. This modification is of particular relevance because it is associated with a variety of cellular control processes, some of which are dysregulated in cancer cells. For example, the source proteins for class I MHC-associated phosphopeptides are often known phosphoproteins, supporting the idea that the phosphopeptides are processed from folded proteins participating in signaling pathways.

Although not wishing to be bound by any particular theory, it is envisioned that in some embodiments the target peptides of the presently disclosed subject matter are unexpectedly superior than known tumor-associated antigen-derived peptides for use in immunotherapy because: 1) they only displayed on the surface of cells in which intracellular phosphorylation is dysregulated (i.e., cancer cells) and not normal thymus cells, and thus they are not compromised by self-tolerance (as opposed to TAAs generally, which are associated with overexpression or otherwise expressed on non-mutated cells); and/or 2) they identify a cell displaying them on their surface as having dysregulated phosphorylation. Thus, post-translationally modified phosphopeptides that are differentially displayed on cancer cells and derived from source proteins objectively linked to cellular transformation and metastasis allow for more extensive anti-tumor responses to be elicited following vaccination. Target peptides are, therefore, better immunogens in peptide-based vaccines, as target peptides are derived from proteins involved with cellular growth control, survival, and/or metastasis, and alterations in these proteins as a mechanism of immune escape might interfere with the malignant phenotype of tumors.

As such, the presently disclosed subject matter also includes in some embodiments methods of identifying target peptides for use in immunotherapy which are displayed on transformed cells but are not substantially expressed on normal tissue in general or in the thymus in particular. In some embodiments, such target peptides bind the MHC class I molecule more tightly than their non-phosphorylated native counterparts. Moreover, such target peptides might have additional binding strength by having amino acid substitutions at certain anchor positions. In some embodiments, such modified target peptides will remain cross-reactive with TCRs specific for native target peptide MHC complexes.

Additionally, it is envisioned that the target peptides associated with proteins involved in intracellular signaling cascades or cycle regulation are of particular interest for use in immunotherapy. In some cases, the TCR might specifically react with the phosphate groups on the target peptide being displayed on an MHC class I molecule.

In some embodiments, a method for screening target peptides for use in immunotherapy (e.g., in adaptive cell therapy or in a vaccine) involves determining whether the candidate target peptides are capable of inducing a memory T cell response. The contemplated screening methods can include providing target peptides (including but not limited to those disclosed herein or those to be identified in the future) to a healthy volunteer and determining the extent to which a target peptide-specific T cell response is observed. In some embodiments, the extent to which the T cell response is a memory T cell response is also determined. In some embodiments, the extent to which a T_(CM) response is elicited, such as but not limited to the extent to which a T_(CM) response is elicited relative to other T cell types, is determined. In some embodiments, those target peptides that are capable of inducing a memory T cell response in healthy and/or diseased patients are selected for inclusion in the therapeutic compositions of the presently disclosed subject matter.

In some embodiments, the presently disclosed subject matter also provides methods for inducing a target peptide-specific memory T cell (e.g., T_(CM)) response in a patient by providing the patient with a composition comprising the target peptides disclosed herein. In some embodiments, the compositions are provided in a dosing regimen as disclosed herein.

In some embodiments, the presently disclosed subject matter also relates to methods for determining a cancer disease prognosis. These methods can involve providing a patient with target peptide compositions and determining the extent to which the patient is able to mount a target peptide-specific T cell response. In some embodiments, the target peptide composition comprises target peptides selected in substantially the same manner that one would select target peptides for inclusion in a therapeutic composition. If a patient is able to mount a significant target peptide specific T cell response, then the patient is likely to have a better prognosis than a patient with the similar disease and therapeutic regimen who is not able to mount a target peptide specific T cell response. In some embodiments, the methods involve determining whether the target peptide specific T cell response is a T_(CM) response. In some embodiments, the presence of a target peptide-specific T cell response as a result of the contemplated diagnostic method correlates with an at least or about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 125, 150, 175, 200, 250, 300, 400, 500 or more percent increase in progression free survival over standard of care.

The above disclosure generally describes the presently disclosed subject matter. A more complete understanding can be obtained by reference to the following specific examples which are provided herein for purposes of illustration only, and are not intended to limit the scope of the presently disclosed subject matter.

EXAMPLES

The following Examples provide further illustrative embodiments. In light of the present disclosure and the general level of skill in the art, those of skill will appreciate that the following EXAMPLES are intended to be exemplary only and that numerous changes, modifications, and alterations can be employed without departing from the scope of the presently disclosed subject matter.

Example 1 MHC Class-I-Associated Phosphopeptides Associated with CRC

Disclosed herein are MHC class-I-associated phosphopeptides associated with colorectal cancer (CRC). CRC tumors and matched normal tissues were lysed, the MHC class-I complexes affinity-purified, and bound peptides eluted. Phosphopeptides were enriched using immobilized metal affinity chromatography and characterized using mass spectrometry. Phosphopeptides were considered tumor specific if their levels in tumor were at least double that of the matched normal tissue.

21 tumor-specific CRC-associated MHC class I phosphopeptides were identified from a liver metastasis. A selection of these phosphopeptides was also found on primary tumors and CRC cell lines. These phosphopeptides derive from proteins that contribute to key CRC-associated oncogenic pathways, including the MAP kinase, interleukin, and p53 signaling pathways.

Tumor infiltrating lymphocytes (“TILs”) from the same tumors, were extracted and expanded, and their responses to the phosphopeptides assessed using multiplexed intracellular cytokine staining for IL-2, IFNγ, and TNFα as depicted in FIG. 1A. Multifunctional TILs were found that responded to the phosphopeptides identified producing IL-2, IFNγ, and TNFα (see FIG. 1B). These responses are summarized in FIG. 1C.

The TILs from the tumor from which the phosphopeptides were identified responded to many more of the phosphopeptides than TILs from other tumors. In particular, there was a significant response (˜2% of TILs, equivalent to the EBV peptide response) to the HLA-B7-associated phosphopeptide from LISCH7, which was also the most abundant phosphopeptide found on the tumor. There were, however, TIL responses from other tumors to some of the tumor-specific phosphopeptides, particularly those MHC class-I-associated phosphopeptides whose HLA specificity is unknown. These phosphopeptides have been found on many tumors from patients of differing HLA-types. Additionally, responses were seen to the phosphopeptide from RASSF6, a key oncoprotein involved in MAPK signaling, suggesting that these oncogenic proteins may be good targets for tumor immunotherapeutics.

Example 2 Healthy Donor PBMC Responses to Exemplary CRC Tumor-Specific Phosphopeptides Assessed by ELISpot

Healthy donor PBMC responses to the CRC tumor-specific phosphopeptides were assessed using a cultured IFNγ ELISpot on day 7. Heterogeneous responses were seen to many of the phosphopeptides from different HLA-matched healthy donors (see FIG. 2). This indicates that these phosphopeptides are good vaccine candidates. In particular, many responses were seen to the RNASE4 phosphopeptide, of unknown HLA-specificity, the HLA-B7 phosphopeptide from ZPF36L2 and strong responses were often seen to the two IRS2 phosphopeptides.

Example 3 Establishment of Phosphopeptide-Specific T Cell Lines and Assays for Ability to Kill CRC Cell Line SW620

T cells lines were established against some of these key phosphopeptide targets. Both the IRS2 (A2) and ZPF36L2 (B7) phosphopeptide-specific cell lines were shown to specifically kill the CRC cell line SW620 (see FIG. 3).

The phosphopeptides disclosed herein are involved in key CRC oncogenic pathways. TILs from the same tumor as that which the phosphopeptides were identified in, respond to a subset of the phosphopeptides. TILs from other tumors also respond to some of these phosphopeptides. The phosphopeptides are also immunogenic in healthy donors, producing heterogeneous responses and are thus good vaccine candidates. Phosphopeptide-specific T cell lines from healthy donors can kill a CRC cell line.

Example 4 T Cell Recognition and Memory Response Assays

Generally, quantification of T cell IFNγ production in response to peptides was performed by ELISpot as per manufacturer's instructions. In brief, ELISpotPRO wells, pre-coated with IFNγ monoclonal antibody mAb 1-D1K (MabTech, product code: 3420-2APW-2), were washed four times with sterile PBS and blocked for 30 minutes with 200 μl 10% RPMI 1640 after which the blocking medium was removed.

For ELISpot analysis, PBMCs and CD8+ T cells were isolated fresh from healthy donors and patients. 1×10⁶ PBMCs were isolated from both disease patients and healthy donors and re-suspended in AIM-V media (10% human AB serum) in a 96 well plate. Peptide or phosphopeptides were added individually at 10 μg/ml and placed at 37° C. in CO₂ incubator for 7 days. Cells were then harvested, washed four times, and re-challenged with either phosphopeptides, peptides, anti CD3 (OKT3, 100 ng/ml, Mabtech) as per manufacturer's instructions. Individual cytokine-producing cells were identified as dark spots after a 15 minute reaction with 5-bromo-4-chloro-3-indolyl phosphate and NBT by means of an alkaline phosphatase conjugate substrate (Mabtech). Spots were counted using an automated reader, (AID-Diagnostika), and results displayed as number of spot-forming cells (SFC) per 10⁶ PBMCs (see FIGS. 2 and 4).

It is envisioned that the T cell activated by the peptides disclosed herein express increased levels of CD45RA and/or CD27 in accordance with the target peptides' ability to stimulate central memory (T_(CM)) T cells.

Example 5 TIL Responses to Tumor-Specific, MHC Class-I Associated Phosphopeptide Discovered in Patient Samples

An exemplary scheme for isolating and characterizing phosphopeptides from CRC tumor samples is depicted in FIG. 5. Summarily, tumor and normal tissue were taken from the same patient at resection. These were each lysed and the MHC Class I complexes affinity purified using pan Class I antibody, W6/32. The MHC Class I-associated peptides were eluted in acid and phosphopeptides enriched using immobilized metal affinity chromatography (IMAC). These phosphopeptides were characterized using CAD and ETD LC-MS/MS and the sequences manually assigned. The phosphopeptides were then synthesized and verified.

Tumor infiltrating lymphocytes (TILs) were also taken from the patient tumors at resection and expanded, using high-dose IL-2 (6000 IU/ml). TIL responses to the phosphopeptides were then tested using ICS. TILs were stimulated with phosphopeptide and cultured for 7 days before being re-stimulated in an intracellular cytokine staining assay assessing production of tumor necrosis factor alpha (TNFα), interferon gamma (IFNγ) and interleukin-2 (IL-2). The results are depicted in FIG. 6.

REFERENCES

All references listed in the instant disclosure, including but not limited to all patents, patent applications and publications thereof, scientific journal articles, and database entries (including but not limited to Uniprot and GENBANK® database entries and including all annotations available therein) are incorporated herein by reference in their entireties to the extent that they supplement, explain, provide a background for, and/or teach methodology, techniques, and/or compositions employed herein. The discussion of the references is intended merely to summarize the assertions made by their authors. No admission is made that any reference (or a portion of any reference) is relevant prior art. Applicants reserve the right to challenge the accuracy and pertinence of any cited reference.

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It will be understood that various details of the presently disclosed subject matter may be changed without departing from the scope of the presently disclosed subject matter. Furthermore, the foregoing description is for the purpose of illustration only, and not for the purpose of limitation.

TABLE 3 Annotation for Protein Sources of Class I MHC Phosphopeptides Presented on Colorectal Cancer Expression Levels Source Predicted Liver Cell Protein HLA Type Metastasis Primary Line CRC Pathway RASSF6 A2 + MPAK signaling DENND4C B7 ++ IRS2 A2 Insulin signaling LSP1 A2 BARD1 A3 ++ DNA damage signaling PDCL A3 + Rab11FIP1 B7 ++ IRS2 A2 +++ Insulin signaling PALLD B7 ++ MUC12 B7 +++ TGFβ signaling AHCYL2 B7 ++ Methionine metabolism TPX2 B7 ++ p53 signaling PDLIM2 B7 ++ IL-12 signaling NUMBL B7 +++ ++ Notch signaling Unidentified B7 ++ ZFP36L2 B7 ++ ++ LISCH7 B7 ++++ YT521 Unknown ++ RNASE4 Unknown +++ ++ FILIP1L Unknown +++ ++ SELH Unknown +++ ++

TABLE 4 Exemplary MHC Class Cancer I Phosphopeptides  Associated with Colorectal Cancer MHC SEQ Source Class I Peptide ID Protein Subtype Sequence NO. RASSF6 A*0301 RTMsEAALVRK 40 DENND4C B*0702 RPFHGISTVsL 69 IRS2 A*0201 RVAsPTSGV 23 LSP1 A*0201 RQAsIELPSMAV 18 BARD1 A*0301 RLSsPISKR 39 PDCL A*0301 SVRRsVLMK 45 Rab11-FIP1 B*0702 KPEsRRSSLL 54 IRS2 A*0201 AVRPTRLsL 93 PALLD B*0702 RPDsAHKML 67 MUC12 B*0702 SPRsPDRTL 87 AHCYL2 B*0702 RPTKIGRRsL 81 TPX2 B*0702 QPQRRsLRL 63 PDZ-LIM2 B*0702 RPGsRQAGL 71 NUMB B*0702 SPFKRQLsL 84 ZFP36L2 B*0702 LPIFSRLsI 60 LISCH7 B*0702 RPRARsVDAL 75 TTC22 B*0702 APRDRRAVsF 50 SELH B*2705 RRGsFEVTL 132 RNASE4 unknown RTHsLLLLL 176 FILIP1L B*2705 RRIsDPQVF 135 YT521 unknown RARGIsPIVF 64

TABLE 5 Exemplary Phosphopeptides Associated with Colorectal Cancer (CRC) SEQ ID Amino NO Sequence Accession^(a) Acids^(b) Source Protein 1 ISSsMHSLY P50616 222-230 Protein Tob1 2 ITQGtPLKY Q9Y618 1459-1467 Nuclear receptor corepressor 2 3 LTDPSsP Q8IX90 341-352 Spindle and kinetochore-associated protein 3 TISSY 4 TMAsPGKDNY O60684  3-12 Importin subunit alpha-7 5 AIsDLQQL O15302 152-159 CaM kinase II isoform 6 DLKRRsMSI Q96N67 175-183 Dedicator of cytokinesis protein 7 7 FLDtPIAKV Q969G9 320-238 Protein naked cuticle homolog 1 8 KAFsPVRSV Q02363  2-10 DNA-binding protein inhibitor ID-2 9 KLAsPELERL P05412 70-79 Transcription factor AP-1 10 KLFPDtPLAL Q12906 587-596 Interleukin enhancer-binding factor 3 11 KLIDRTEsL P33241 197-205 Lymphocyte-specific protein 1 12 KLIDVsSQKV O14757 461-471 Serine; threonine-protein kinase Chk1 13 KVQsLRRAL Q969G5 185-193 PRKCDBP; Protein kinase C delta-binding protein 14 LLLsEEVEL Q8IY92 489-497 Structure-specific endonuclease subunit SLX4 15 RLAsYLDRV P05783 90-98 Keratin, type I, cytoskeletal 18 16 RLSsPLHFV Q8NC44 400-408 Protein FAM134A 17 RQAsIELPSM P33241 249-258 Lymphocyte-specific protein 1 18 RQAsEEL P33241 249-260 Lymphocyte-specific protein 1 PSMAV 19 RQDsTPGKVFL P13056 61-71 Nuclear receptor subfamily 2 group C member 1 20 RQDStPGKVFL P13056 61-71 Nuclear receptor subfamily 2 group C member 1 21 RQIsQDVKL Q01433 165-173 AMP deaminase 2 22 RTLsPEIITV Q9H4A3 1802-1812 Serine; threonine-protein kinase WNK1 23 RVAsPTSGV Q9Y4H2 1097-1105 Insulin receptor substrate 2; IRS2 24 RVLHsPPAV Q9Y4B5 1510-1518 SOGA2; coiled-coil domain-containing protein 165 25 SMTRsPPRV Q9BRL6 248-256 Serine; arginine-rich splicing factor 8 26 SVKPRRTsL P15822 766-774 Zinc finger protein 40 27 VMIGsPKKV Q68CZ2 1437-1445 Tensin-3 28 RADsPVHM O95402 444-451 Mediator of RNA polymerase II transcription subunit 26 29 RAHSsPASL P46937 124-132 Yorkie homolog; 65 kDa Yes-associated protein; YAP65  30 RIsHELDS P10451 301-308 Osteopontin 31 RSHSsPASL Q9GZV5 86-94 WW domain-containing transcription regulator protein 1 32 YAVPRRGsL O95425  993-1001 Supervillin 33 GIMsPLAKK Q03989 253-262 AT-rich interactive domain-containing protein 5A 34 KLPsPAPARK Q8IY33 140-149 MICALL2; MICAL-Iike protein 2 35 RAKsPISLK Q9BXL7 509-517 Caspase recruitment domain-containing protein 11; CARD-containing MAGUK protein 1; CARMA1 36 RILsGVVTK P62280 71-79 40S ribosomal protein S11 37 RIYQyIQSR Q9Y463 269-277 Dual specificity tyrosine-phosphorylation- regulated kinase 1B 38 RIYQyIQSRF Q9Y463 269-278 Dual specificity tyrosine-phosphorylation- regulated kinase 1B 39 RLSsPISKR Q99728 327-335 BARD1; BRCA1-associated RING domain protein 1 40 RTMsEAALVRK Q6ZTQ3 184-194 RASSF6; RASF6; Ras association domain- containing protein 6 41 RTRsLSSLREK O94915 1975-1985 FRYU; FRYL; Protein furry homolog-like 42 RVAsPTSGVK Q9Y4H2 1097-1106 Insulin receptor substrate 2; IRS2 43 RVLsPLIIK Q8NCN4 400-408 E3 Ubiquitin-protein ligase RNF169 44 RVYsPYNHR Q9NS56 582-590 E3 Ubiquitin-protein ligase Topers 45 SVRRsVLMK Q9H2J4 223-231 PDCL3; Phosducin-like protein 3 46 KRAsVFVKL P50502 153-161 Hsc70-interacting protein 47 SVKsPVTVK Q9HCS4 329-337 Transcription factor 7-like 1 48 INKERRSsL Q5JTZ5 81-89 Uncharacterized protein C9orf152 49 APDsPRAFL — — No data base hit 50 APRDRRAVsF Q5TAA0 560-569 TTC22; Tetratricopeptide repeat protein 22 51 APRRYsSSL Q68EM7 697-705 ARHGAP17; RGH17; Rho GTPase-activating protein 17 52 GPRsPKAPP Q6PJ34 313-321 ARHGAP4 protein 53 KPAsPKFIVTL Q6PJT7 512-522 Zinc finger CCCH domain-containing protein 14 54 KPEsRRSSLL Q6WKZ4 428-437 RAB11-FIP1; Rab 11 family-interacting protein 1 55 KPRPPPLsP Q15642; 328-336 Cdc42-interacting protein 4; CIP4 XP_  005259738 56 KPRsPFSKI Q9BXF6 185-193 RAB11-FIP5; RFIP5; Rab11 family- interacting protein 5 57 KPRsPPRAL Q86TG8; 249-257 Retrotransposon-derived protein P07288; PEG10 isoform 2 NP_ 001035242 58 KPRsPVVEL P25098 667-675 Beta-Adrenergic receptor kinase 1 59 LPAsPRARL Q3KQU3 443-451 Map 7 domain-containing protein 1 60 LPIFSRLsI P47974 483-491 ZFP36L2; Zinc finger protein 36,  C3H1 type-like 2 61 LPVsPRLQL P40199 185-193 Carcinoembryonic antigen-related cell adhesion molecule 6 62 MPRQPsATRL Q6P582 134-143 Mitotic-spindle organizing protein 2A 63 QPQRRsLRL Q9ULW0 116-124 TPX2; Targeting protein for Xklp2 64 RARGIsPIVF Q96MU7 303-312 YTHDC1; YTDC1; YTH domain-containing protein 1; YT521 65 RPAsAGAML Q14814 198-206 Monocyte-specific enhancer factor 20 66 RPAsPQRAQL — — No data base hit 67 RPDsAHKML B7ZMM5 969-977 PALLD; Palladin protein 68 RPDVAKRLsL O75815 282-291 BCAR3; Breast cancer anti-estrogen resistance protein 3 69 RPFHGISTVsL Q5VZ89 1417-1427 DENND4C; DENN domain-containing protein 4C 70 RPFsPREAL Q86V48 742-750 Leucine zipper protein 1 71 RPGsRQAGL Q96JY6 175-183 PDZ-LIM2; PDZ and LIM domain protein 2 72 RPKsPLSKM Q9HCD6 1576-1584 TANC2; Tetratricopeptide repeat 73 RPKsVDFDSL Q9Y5K6 455-464 CD2AP; CD2-associated protein 74 RPQRATsNVF P19105 13-22 Myosin regulatory light chain 12A 75 RPRARsVDAL Q9BT33; 426-435 LSR Protein; Lipolysis-stimulated Q86X29 lipoprotein receptor; LISCH7 76 RPRGsQSLL P21860 1040-1047 Receptor tyrosine-protein kinase erbB-3 77 RPRPVsPSSL P57059 430-439 Serine/threonine-protein kinase SIK1 78 RPRsAVLL Q12802 1873-1880 A-kinase anchor protein 13; AKAP-13 79 RPRsPNMQDL Q6T310 214-223 RASL-11A; Ras-like protein family member 11A 80 RPRsPRQNSI Q99700 689-698 Ataxin-2 81 RPTKIGRRsL Q96HN2 135-144 AHCYL2; SAHH3; Putative adenosylhomocysteinase 3 82 RPTsRLNRL Q15788 860-868 NCoA1; Nuclear receptor coactivator 1 83 RPVtPVSDL Q13118 63-71 KLF10; Krueppel-like factor 10 84 SPFKRQLsL P49757 288-296 NUMB; Numb protein homolog 85 SPRAPVsPLKF Q9UBS0 417-427 RPS6KB2; Ribosomal protein S6 kinase beta-2 86 SPRRsRSISL Q16629 159-168 Serine/arginine-rich splicing factor 7 87 SPRsPDRTL Q9UKN1 286-294 MUC12; Mucin-12 88 SPRSPsTTYL Q13111 772-781 Chromatin assembly factor 1 subunit A 89 SPRsPSTTYL Q13111 772-781 Chromatin assembly factor 1 subunit A 90 TPRsPPLGL Q16584 755-763 Mitogen-activated protein kinase kinase kinase 11 91 VPRPERRsSL Q6UWJ1 668-677 TMCO3; Transmembrane and coiled-coil domain- containing protein 3 92 APRKGsFSAL Q13619  5-14 Cullin-4A 93 AVRPTRLsL Q9Y4H2 887-895 Insulin receptor substrate 2; IRS2 94 KPRPLsMDL Q9BY89 279-287 Uncharacterized protein KIAA1671 95 KPRRFsRsL Q7L4I2 209-217 Arginine/serine-rich coiled-coil protein 2 96 LPRtPRPEL Q8N1W2 174-182 Zinc finger protein 710 97 RPAsPAAKL Q9P2N6 512-520 KIAA1310; KAT8 regulatory NSL complex subunit 3 98 RPDsPTRPTL Q7RTP6 1646-1655 Protein-methionine sulfoxide oxidase MICAL3 99 RPIsPRIGAL Q9Y6I3;  93-102 Epsin-1 NP_ 001123543 100 RPRAAtW P10644 333-340 cAMP-dependent protein kinase type 1- alpha regulatory subunit 101 RPRAAtWA P10644 333-341 cAMP-dependent protein kinase type 1- alpha regulatory subunit 102 RPRANsGGVDL Q92766 1162-1172 Ras-responsive element-binding protein 1 103 RPRsAVEQL Q9HAU0 882-890 Pleckstrin homology domain- containing family A member 5 104 RPRsMTVSA O43312 457-465 Metastasis suppressor protein 1 105 RPRsPPGGP Q86UZ6 573-581 Zinc finger and BTB domain-containing protein 46 106 RPRsPT Q9HAU0 882-890 Pleckstrin homology domain- GPSNSF containing family A member 5 107 SPKsPGLKA B7ZKW8; 75-83 CapZ-interacting protein isoform XI XP_ 005245662 108 YPGGRRsSL P22897 1037-1045 Macrophage mannose receptor 1 109 KYIsGPHEL P49454 1270-1279 Centromere protein F 110 RYQtQPVTL O95425 849-857 Supervillin 111 FRRsPTKSSL Q96PK6 624-633 RNA-binding protein 14 112 FRRsPTK Q96PK6 624-635 RNA-binding protein 14 SSLDY 113 GRKsPPPSF B4DLE8; 713-721 Beta/gamma crystallin domain-containing NP_705833 protein 3 114 GRLsPAYSL Q86UU1 536-544 Pleckstrin homology-like domain family B member 1 115 GRLsPVPVPR Q9UKM9 132-141 RNA-binding protein Raly 116 HRLsPVKGEF Q9Y2L9 367-376 Leucine-rich repeat and calponin homology  domain-containing protein 1 117 HRNsMKVFL Q9NPR2 735-743 Semaphorin-4B 118 KRFsFKKSF P29966 156-164 Myristoylated alanine-rich C-kinase substrate 119 KRFsFKKsF P29966 156-164 Myristoylated alanine-rich C-kinase substrate 120 KRFsGTVRL P62906 47-55 RPL10N 60S ribosomal protein L10a; NEDD-6 121 KRLsPAPQL Q9UH99 51-59 SUN domain-containing protein 2 122 KRLsVERIY P11388 26-34 DNA Topoisomerase 2-alpha; TOP2A 123 KRMsPKPEL P41208 17-25 Centrin-2 124 KRYsGNMEY O95835 275-283 Serine/threonine-protein kinase LATS1 125 RRAsLSEIGF Q00537 177-186 Cyclin-dependent kinase 17 126 RRAsQEANL Q6PJG2 458-466 Uncharacterized protein C14orf43; LM2 and SANT domain-containing protein 1 127 RRDsIVAEL O14579 96-104 Coatomer subunit epsilon 128 RRDsLQKPGL Q9NRM7 376-386 Serine/threonine-protein kinase LATS2 129 RRFsGTAVY Q6AHZ1 652-660 ZNF518N Zinc linger protein 518A 130 RRFsPPRRM Q15287 248-256 RNA-binding protein with serine-rich domain 1 131 RRFsRLENRY O43293 411-420 Death-associated protein kinase 3 132 RRGsFEVTL Q8IZQ5; 75-83 Protein C11orf31; selenoprotein H; SELH NP_734467 133 RRIDlsPSTF Q9Y2W1 677-686 Thyroid hormone receptor-associated protein 3 134 RMDIsPSTLR Q9NYF8 653-663 Bcl-2-associated transcription factor 1 135 RRIsDPQVF Q4L180 788-796 FILIP1L; Filamin A-interacting protein 1-like 136 RRIsGVDRY O15239 52-60 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 137 RRIsGVDRYY O15239 52-61 NADH dehydrogenase [ubiquinone)1 alpha subcomplex subunit 1 138 RRKsQVAEL Q9BYG3 244-252 MKI67 FHA domain-interacting nucleolar phosphoprotein 139 RRLsADIRL O60307 744-752 Microtubule-associated serine/threonine-protein kinase 3 140 RRLsDSPW P47974 435-443 Zinc finger protein 36, C3H1 type-like 2 141 RJlLsESSAL Q96S55 72-80 ATPase WRNIP1 142 RRLsGPLHTL Q86Y91 610-619 Kinesin-like protein KIF18B 143 RRLsLFLNV Q99836 31-39 Myeloid differentiation primary response protein My088 144 RRMsFQKP Q8N573 88-95 Oxidation resistance protein 1 145 RRMsLLSVV Q9ULI2 314-322 Beta-citryl-glutamate synthase B 146 RRNsAPVSV Q2M1Z3 1175-1183 Rho GTPase-activating protein 31 147 RRNsINRNF O00160 731-739 Unconventional myosin-1f 148 RRPsLLSEF O75376 67-75 Nuclear receptor corepressor 1 149 RRQsKVEAL Q03169 120-128 TNFAIP2; tumor necrosis factor alpha-induced protein 2 150 RRLsELLRY P08238 449-457 Heat shock Protein HSP 90-beta 151 RRLsFLVSY P47897 67-75 Giutamine-tRNA ligase 152 RRSsFLQVF Q15436 585-593 Protein transport protein Sec23A 153 RRSsIQSTF Q92542 232-240 Nicastrin 154 RRsSIQSTF Q92542 232-240 Nicastrin 155 RRSsSVAQV O15205 107-115 Ubiquitin D 156 RRYsPPIQ AAP97290; 592-599 Ser/Arg repetitive nuclear matrix protein 1 AF419855 157 VRAsKDLAQ 60563 164-172 CCNT1; Cyclin-T1 158 KRLEKsPSF Q92625 656-664 Ankyrin repeat and SAM domain-containing protein 1A 159 KRLEKSPsF Q92625 656-664 Ankyrin repeat and SAM domain-containing protein 1A 160 KRWQsPVTK XP_ 518-526 Serine/arginine repetitive matrix protein 1 isoform X6 005245779 161 RRFsIATLR Q16696 128-136 Cytochrome P450 2A13 162 RRFsLTTLR P10632 124-132 Cytochrome P450 2C8 163 RRFsRsPIR P18583 2026-2034 Protein SON isoform B 164 RRFsVSTLR P10635 132-140 Cytochrome P450 2D6 165 RRFsVTTMR P20813 125-133 Cytochrome P450 2B6 166 RRVVQRSsL Q04637 1098-1105 Eukaryotic translation initiation factor 4 gamma 1 isoform 2 167 RRYsPPIER Q8IYB3 594-602 Serine/arginine repetitive matrix protein 1 168 RRYsPPIQR Q8IYB3 594-602 Serine/arginine repetitive matrix protein 1 169 AENsPTRQQF Q86XP3  93-102 ATP-dependent RNA helicase DDX42 170 EELsPTKAF Q99612 117-125 Krueppel-like factor 6 171 KEMsPTRQL Q4G0N7 36-44 UPF0731 protein C6orf225; protein FAM229B 172 RFKtQPVTF Q7Z7L8 365-373 Uncharacterized protein C11orf96 173 EERRsPPAP P15408 196-204 Fos-related antigen 2 174 FEDDDsNEKL O43719 697-706 HIV Tat-specific factor 1 175 KsLVRLLLL P07998  5-13 Ribonuclease pancreatic 176 RTHsLLLLL P34096  5-13 RNASE4; Ribonuclease 4 177 RTSSFtFQN P27540 440-448 Aryl hydrocarbon receptor nuclear translocator; ARNT ^(a)Refers to the Uniprot and/or GENBANK® biosequence database accession number(s). ^(b)Refers to the amino acid positions for the peptide in the corresponding Uniprot and/or GENBANK© biosequence database accession(s).

TABLE 6 Characteristics of HLA-DR-associated Phosphopeptides Selectively  Expressed by Melanoma Cells SEQ ID Source Protein Phosphopeptide NO 1363-mel and ₁₀₀APPAYEKLsAEQ₁₁₁ 218 2048-mel ₁₀₀APPAYEKLsAEQSPP₁₁₄ 219 Melanoma ₁₀₀APPAYEKLsAEQSPPP₁₁₅ 220 antigen ₁₀₀APPAYEKLsAEQSPPPY₁₁₆ 226 recognized by T cells- 1/MART-1 Tensin-3 ₁₄₃₄VSKVMIGsPKKV₁₄₄₅ 227 ₁₄₃₇VMIGsPKKV₁₄₄₅ 228 1363-mel alone ₁₄₂KYsPGKLRGN₁₅₁ 229 Matrix- remodeling- Associated Protein 7 2048-mel  ₁₇₆SKEDKNGHDGDTHQ 230 alone Amino- EDDGEKsD₁₉₇ terminal enhancer of split Ankyrin  ₄₃GSALGGGGAGLSGRASGG 231 repeat AQsPLRYLHV₇₁ domain- ₄₆LGGGGAGLSGRASGG 232 containing AQsPLRYLHV₇₁ protein-54 ₅₈SGGAQsPLRYLHVL₇₂ 233 Anoctamin-8 ₆₃₈EEGsPTMVEKGLEPGVFTL₆₅₆ 234 ₆₃₉EGsPTMVEKGLEPGVFEU₆₅₆ 235 ₆₄₀GsPTMVEKGLEPGVFTL₆₅₆ 236 AP-3 complex ₇₇₉EEMPENALPsDEDD 237 subunit-Δ-1 KDPNDPYRAL₈₀₂ Casein ₂₀₂QAASNFKsPVKTIR₂₁₅ 238 kinase II ₂₀₃AASNFKsPVKTIR₂₁₅ 239 subunit-β ₂₀₅SNFKsPVKTIR₂₁₅ 240 ₂₀₆NFKsPVKTK₂₁₅ 241 ₂₀₇FKsPVKTIR₂₁₅ 242 Claudin-11 ₁₉₁YYTAGSSsPTHAKSAHV₂₀₇ 243 ₁₉₆SSsPTHAKSAHV₂₀₇ 244 Emerin ₁₁₇VRQsVTSFPDADAFHHQ₁₃₃ 245 FLJ20689 ₄₇₁FKMPQEKsPGYS₄₈₂ 246 Insulin  ₁₀₉₇RVAsPTSGVKR₁₁₀₇ 247 receptor Substrate 2 Interleukin 1 ₅₄₃QVAMPVKKSPRRSSS 248 receptor DEQGLSYSSLKNV₅₇₀ accessory ₅₄₄VAMPVKKSPRRSSSDE 249 protein QGLSYSSLKNV₅₇₀ LUC7-like  ₃₅₃SSNGKMASRRsEEKEAG₃₆₉ 250 isoform b ₃₅₃SSNGKMASRRSEEKEAGEI₃₇₁ 251 Membrane- ₁₇₂KEGEEPTVYsDEE 252 associated EPKDESARKND₁₉₅ progesterone ₁₇₃EGEEPTVYsDEEE 253 receptor PKDESARKND₁₉₅ component 1 NF-κB inhibitor- ₁₆₅ASKMTQPQSKSAFPLS 254 Interacting RKNKGsGsLDG₁₉₁ Ras-like protein 2 Probable  ₃₄₈APPPLVPAPRPSsPP 255 fibrosin-1 RGPGPARADR₃₇₂ long transcript  protein isoform 2 Small acidic ₂SAARESHPHGVKRSAsPDDDLG₂₃ 256 protein ₂(AcS)AARESHPHGVKRS 257 AsPDDDLG₂₃ Synaptojanin-170 ₁₅₆₁ASKAsPTLDFTER₁₅₇₃ 258 Tetraspanin-10 ₄GERsPLLSQETAGQKP₁₉ 259 ₄GERsPLLSQETAGQKPL₂₀ 260 ₅ERsPLLSQETAGQKP₁₉ 261 ₅ERsPLLSQETAGQKPL₂₀ 262 Transmembrane ₄₂₄TIGEKKEPsDKSVDS₄₃₈ 263 protein 184

TABLE 7 Characteristics of HLA-DR-associated Phosphopeptides Selectively Expressed by EBV-transformed B Cells SEQ ID Source Protein Phosphopeptide NO. B lymphocyte ₂₅SGPKPLFRRMsSLVGPTQ₄₂ 264 antigen CD20 ₂₆GPKPLFRRMsS₃₆ 265 ₂₆GPKPLFRRMsSL₃₇ 266 ₂₆GPKPLFRRMsSLV₃₈ 267 ₂₆GPKPLFRRMsSLVG₃₉ 268 ₂₆GPKPLFRRMsSLVGP₄₀ 269 ₂₆GPKPLFRRMsSLVGPT₄₁ 270 ₂₆GPKPLFRRMsSLVGPTQ₄₂ 271 ₂₆GPKPLFRRMsSLVGPTQS₄₃ 272 Lymphoid-restricted ₁₃₀AsPTIEAQGTSPAHDN₁₄₅ 273 membrane protein ₁₃₀AsPTIEAQGTSPAHDNI₁₄₆ 274 ₁₃₀AsPTIEAQGTSPAHDNIA₁₄₇ 275 ₄₀₂SSsWRILGSKQSEHRP₄₁₇ 276 1363-EBV alone ADAM 8 ₇₅₈SPPFPVPVYTRQAPKQVIK₇₇₆ 277 B lymphocyte ₃₂₈DPTRRFFKVtPPPGSGPQ₃₄₅ 278 antigen CD19 Germinal center B _(142/76)RSPEDEYEL 279 cell-Expressed LMPHRISSH_(159/93) transcript 2 _(143/77)SPEDEYELLMPH 280 protein RISSH_(159/93) _(143/77)SPEDEYELLMPH 281 RIsSH_(159/93) _(149/83)ELLMPHRIsSHF_(160/94) 282 _(149/83)ELLMPHRIsSHFL_(161/95) 283 Interleukin-2 ₂₈₂TPDPSKFFSQLsSEHGGDV₃₀₀ 284 receptor subunit-⁵ ₂₈₂TPDPSKFFSQLsSEHGGDVQ₃₀₁ 285 Optineurin ₄₇₃sDFHAERAAREK₄₈₄ 286 Phosphoglycerate ₂₀₃SPERPFLAILGGAKVADK₂₂₀ 287 kinase 1 ₂₀₃SPERPFLAILGGAKVADKIQ₂₂₂ 288 Solute carrier ₁₀₅₀TKDKYMASRGQKAKsMEG₁₀₆₇ 289 family 12, member 6, isoform a TNFAIP3-interacting ₅₅₉VPHHGFEDWsQIR₅₇₁ 290 protein 1 Tumor necrosis factor _(513/50)KIEKIyIMK 291 ADTVIVG_(528/65) receptor superfamily _(514/51)IEKIyIMKADTVIVG_(528/65) 292 member 8 UPF0501 protein ₁₃₆EESsDDGKKY₁₄₅ 293 KIAA1430 Xenotropic and ₆₅₇KNRsWKYN₆₆₄ 294 polytropic ₆₅₇KNRsWKYNQ₆₆₅ 295 retrovirus ₆₅₇KNRsWKYNQSISLR₆₇₀ 296 receptor 1 ₆₅₇KNRsWKYNQSISLRRP₆₇₂ 297 ₆₅₈NRsWKYNQSISLR₆₇₀ 298 ₆₅₈NRsWKYNQSISLRRP₆₇₂ 299 ₆₅₉RsWKYNQSISLRRP₆₇₂ 300 2048-EBV alone BCL2-associated ₆₅₃RRIDIsPSTLR₆₆₃ 134 transcription factor 1 Caspase recruitment ₆₅₃RRIDIsPSTLRK₆₆₄ 301 domain-containing ₅₀₉RAKsPISLK₅₁₇ 35 protein 11 Chromatin-modifying _(49/177)ESsVRSQEDQLSR_(61/189) 302 protein 1a _(49/177)ESsVRSQEDQLSRR_(62/190) 303 Interleukin-10 ₂₉₃DKLSVIAEDSESGKQ₃₀₇ 304 receptor-5 chain ₂₉₃DKLsVIAEDSESGKQN₃₀₈ 305 ₂₉₃DKLsVIAEDSESGKQNP₃₀₉ 306 ₂₉₃DKLSVIAEDSESGKQNPG₃₁₀ 307 ₂₉₃DKLsVIAEDSESGKQNPGDS₃₁₂ 308 ₂₉₄KLsVIAEDSESGKQN₃₀₈ 309 ₂₉₄KLsVIAEDSESGKQNP₃₀₉ 310 ₂₉₄KLsVIAEDSESGKQNPG₃₁₀ 311 NADH-ubiquinone ₈₈NLELSKFRMPQPSSGREsPRH₁₀₈ 312 oxidoreductase ₉₁LSKFRMPQPSSGREsPRH₁₀₈ 313 flavoprotein 3 Protein FAM40A ₃₁₈PPLPEDSIKVIRN 314 MRAAsPPA₃₃₈ Ras association _(184/152/140)RTMsEAA 40 domain LVRK_(194/162/150) containing protein 6 SH2 domain ₈₀MPRPsIKKAQNSQAARQ₉₆ 315 containing 3C isoform 1 Taxi-binding ₁₀₆THKGEIRGASTPFQFRAssP₁₂₅ 316 protein 1, ₁₀₇HKGEIRGASTPFQFRAssP₁₂₅ 317 isoform 1 or 2 UPF0492 protein ₃₉₁STIQNSPTKK₄₀₀ 318  C20orf94

TABLE 8 Characteristics of FILA-DR-associated Phosphopeptides Commonly Expressed by Melanoma and EBV-B Cells SEQ ID Source Protein Phosphopeptide NO Elongin A ₁₂₂RSYsPDHRQK₁₃₁ 319 Ferritin ₁₇₁FDKHTLGDsDNES₁₈₃ 320 heavy chain 321 Frizzled-6 ₆₁₇EPAsPAAsISRLSGEQVDGKG₆₃₇ ₆₂₀SPAASISRLsGEQVDGKG₆₃₇ 322 ₆₂₃ASISRLsGEQVDGKG₆₃₇ 323 ₆₂₃AsISRLsGEQVDGKG₆₃₇ 324 ₆₂₃AsISRLSGEQVDGKG₆₃₇ 325 Insulin like ₂₃₉₂TTKsVKALSSLHG₂₁₀₄ 326 growth ₂₃₉₂TTKsVKALSSLHGDD₂₄₀₆ 327 factor 2 ₂₃₉₂TTKsVKALSSLHGDDQ₂₄₀₇ 328 receptor ₂₃₉₂TTKsVKALSSLHGDDQD₂₄₀₈ 329 ₂₃₉₂TTKsVKALSSLHGDDQDS₂₄₀₉ 330 ₂₃₉₃TKsVKALSSLHGDD₂₄₀₆ 331 ₂₃₉₃TKsVKALSSLHGDDQ₂₄₀₇ 332 ₂₃₉₃TKsVKALSSLHGDDQD₂₄₀₈ 333 ₂₃₉₄KsVKALSSLHGDDQ₂₄₀₇ 334 ₂₃₉₄KsVKALSSLHGDDQD₂₄₀₈ 335 ₂₃₉₂TTKSVKALSSLHGDDQDsED₂₄₁₁ 336 ₂₃₉₂TTKSVKALSSLHGDDQDsEDE₂₄₁₂ 337 ₂₃₉₄KSVKALSSLHGDDQDsEDE₂₄₁₂ 338 ₂₄₇₆KLVSFHDDSDEDL₂₄₈₈ 339 Lipolysis- _(324/287/305)APSTYAHLs 340 stimulated PAK_(335/398/316) lipoprotein _(324/287/305)APSTYAHLsPAKTP 341 receptor PPP_(340/303/321) Plakophilin-4 ₂₀₆NRAMRRVsSVPSR₂₁₅ 342 ₂₀₆NRAMRRVsSVPSRAQ₂₂₀ 343 ₂₇₈RPAsPtAIRRIGSVTSRQT₂₉₆ 344 Sequestosome-1 ₃₃₂SGGDDDWTHLSSKEVDPST₃₅₀ 345 ₃₃₂SGGDDDWTHLSSKEVDPSTG₃₅₁ 346 ₃₃₂SGGDDDWTHLSSKEVDPSTGE₃₅₂ 347 ₃₃₂sGGDDDWTHLSSKEVDPSTGEL₃₅₃ 348 ₃₃₂sGGDDDWTHLSSKEVDPSTGELQ₃₅₄ 349 ₃₃₃GGDDDWTHLsSKEVDPS₃₄₉ 350 ₃₃₃GGDDDWTHLsSKEYDPSTG₃₅₁ 351 ₃₃₄GDDDWTHLsSKEVD₃₄₇ 352 ₃₃₄GDDDWTHLSSKEVDP₃₁₈ 353 ₃₃₄GDDDWTHLSSKEVDPS₃₄₉ 354 ₃₃₄GDDDWTULSSKEVDPSTG₃₃₁ 355 ₃₃₅DDDWTHLSSKEVDPS₃₄₉ 356 ₃₃₅DDDWTHLSSKEVDPSTG₃₅₁ 357 ₃₃₆DDWTHLsSKEVDPS₃₄₉ 358 ₃₃₇DWTHLSSKEVDPS₃₄₉ 359 ₃₃₇DWTHLsSKEVDPSTG₃₅₁ 360 ₃₃₈WTHLsSKEVDPS₃₄₉ 361 ₃₃₈WTHLsSKEVDPSTG₃₅₁ 362 Sorting nexin-17 ₄₀₂GtLRRSDSQQAVK₄₁₄ 363 ₄₀₃GtLRRSDSQQAVKS₄₁₅ 364 ₄₀₂GtLRRSDSQQAVKSPP₄₁₇ 365 UPF0555 protein ₇₈₃VLKSRKSSVTEE₉₄ 366 KIAA0776

TABLE 9 Characteristics of Source Proteins Generating HLA-DR-restricted Phosphopeptides GENBANK ® Known Source protein Accession No. Phosphoprotein? Function Other Names ADAM 8 P78325 N Cellular trafficking A disintegrin and metalloproteinase domain 8, Cell surface antigen MS2, CD 156a Amino-terminal enhancer Q08117 N Transcriptional GRG protein, Protein ESP1, Gp130-associated protein of split regulation GAM Ankyrin repeat domain- Q6NXT1 Y Unknown N/A containing protein 54 Anoctamin-8 Q9HCE9 N Ion transport Transmembrane protein 16H AP-3 complex subunit- O14617 Y Trafficking Adapter-related protein complex 3 subunit-A-1, AP-3 A-1 complex subunit-Δ, Δ-adaptin BCL2-associated A2RU75 N Transcription factor N/A transcription factor 1 B lymphocyte antigen P15391 Y Receptor/signal Differentiation antigen CD19, B lymphocyte surface CD19 transduction antigen B4, Leu-12 B lymphocyte antigen P11836 Y Receptor/signal Membrane-spanning 4-domains subfamily A member CD20 transduction 1, B lymphocyte surface antigen 81, Leu-16, Bp35 Casein kinase II subunit- P67870 Y Signal transduction Phosvitin, G5a ll Caspase recruitment Q9BXL7 N Signal transduction CARD-containing MAGUK protein 3, Carma 1 domain-containing protein 11 Chromatin-modifying Q9HD42 N Cell cycle/protein Chromatin-modifying protein 1a, Vacuolar protein protein 1a trafficking sorting-associated protein 46-1 Claudin-11 O75508 N Cell adhesion Oligodendrocyte-specific protein Elongin A Q14241 Y Transcriptional Transcription elongation factor B polypeptide 3, RNA regulation polymerase II transcription factor Sill subunit A 1, SIII p110, Elongin 110-kDa subunit Emerin P50402 Y Protein binding N/A Ferritin heavy chain P02794 Y Ion storage Cell proliferation-inducing gene 15 protein FU20689 Q9H3M3 N Unknown N/A Frizzled-6 O60353 Y Receptor/Signal N/A transduction Germinal center B-cell- Q8N6F7 Y Signal transduction• Germinal center-associated lymphoma protein expressed transcript 2 protein Insulin-like growth factor P11717 Y Metabolism Cation-independent mannose-6-phosphate receptor, 2 receptor M6P/IGF2 receptor, 300 kDa mannose 6- phosphate receptor, CD222 Insulin receptor substrate 2 Q9Y4H2 Y Receptor/signal N/A transduction lnterleukin 1 receptor Q9NPH3 N Receptor/signal N/A accessory protein transduction lnterleukin-2 receptor P14784 Y Receptor/signal High-affinity ll-2 receptor subunit-β, P70-75, CD122 subunit-ll transduction lnterleukin-10 receptor-β Q08334 N Receptor/signal ll-10R2, Cytokine receptor family 2 member 4, chain transduction CDw210b Lipolysis-stimulated Q86X29 Y Receptor/metabolism N/A lipoprotein receptor LUC7-Iike isoform b Q53G47 Y Unknown N/A Lymphoid-restricted Q12912 N Metabolism Protein Jaw1 membrane protein Matrix-remodeling- P84157 N Unknown Transmembrane anchor protein 1 associated protein 7 Melanoma antigen Q16655 N Unknown MART-1, Melan-A, Antigen SK29-AA, Antigen recognized by T cells 1 LB39-AA Membrane-associated O00264 Y Receptor N/A progesterone receptor component 1 NADH-ubiquinone P56181 N Metabolism NADH-ubiquinone oxidoreductase 9-kDa subunit, oxidoreductase Complex 1-9 kD, Renal carcinoma antigen NY- flavoprotein 3 REN-4 Nf-KB inhibitor- Q9NYR9 N Signal regulation IκB-interacting Ras-like protein 2 interacting Ras-like protein 2 Optineurin Q96CV9 Y Signal transduction/ Optic neuropathy-inducing protein, E3-14.7K- protein trafficking interacting protein, FIP-2, Huntingtin-interacting protein L, Huntingtin yeast partner L, NEMO- related protein, Transcription factor IIIA- interacting protein Phosphoglycerate kinase 1 P005658 Y Metabolism Primer recognition protein 2, Cell migration-inducing gene 10 protein Plakophilin-4 Q99569 Y Cell adhesion p0071 Probable fibrosin-1 long- Q9HAH7 Y Unknown N/A transcript protein isoform 2 Protein FAM40A QSVSL9 Y Unknown N/A Ras association domain- Q6ZTQ3 N Signal transduction N/A containing protein 6 Sequestosome-1 Q13501 Y Signal transduction Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa SH2 domain containing QBN5H7 Y Signal transduction Novel SH2-containing protein 3 3C isoform 1 Small acidic protein O00193 Y Unknown N/A Solute carrier family 12, Q9UHW9 Y Ion transport Electroneutral potassium chloride cotransporter 3, KCI member 6, isoform a cotransporter 3 Sorting nexin-17 Q15036 Y Trafficking* N/A Synaptojanin-170 O43426 Y Metabolism Synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1 Tax1-binding protein 1, Q86VP1 N Signal transduction TRAF6-binding protein isoform 1 or 2 Tensin-3 Q68CZ2 Y Cellular structure Tumor endothelial marker 6, Tensin-like SH2 domain- containing protein 1 Tetrasporin-10 Q9H1Z9 N Unknown Oculospanin TNFAIP3 interacting Q15025 Y Signal transduction Nef-associated factor 1, HIV-1 Net-interacting protein, protein 1 Virion-associated nuclear shuttling protein, Nip40-1 Transmembrane protein Q9NVA4 N Unknown Transmembrane protein 34 184C Tumor necrosis factor P28908 N Receptor/signal CD30L receptor, Lymphocyte activation antigen receptor superfamily transduction CD30, Kl-1 antigen, CD30 member 8 UPF0492 protein Q5VYV7 N Unknown N/A C20orf94 UPF0501 protein Q9P2B7 Y Unknown N/A KIAA1430 UPF0555 protein O94874 Y Unknown N/A KIAA0776 Xenotropic and Q9UBH6 Y Receptor/signal Protein SYG 1 homolog, Xenotropic and polytropic polytropic retrovirus transduction murine leukemia virus receptor X3 receptor 1 

1-41. (canceled)
 42. A method of making a vaccine comprising synthesizing at least one target peptide with an amino acid sequence selected from the group consisting of: SEQ ID NO: 3, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 5, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 6, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 7, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 12, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 13, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 14, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 15, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 20, wherein the threonine at the fifth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 22, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 24, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 26, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 28, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 29, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 30, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 31, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 32, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 33, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 34, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 43, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 48, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 49, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 50, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 52, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 54, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 56, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 61, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 63, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 67, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 68, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 71, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 72, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 73, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 81, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 85, wherein the serine at the seventh position of is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 87, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 91, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 93, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 94, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 95, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine, the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine, or any combination thereof; SEQ ID NO: 96, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 99, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 100, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 101, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 103, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 104, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 105, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 106, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 108, wherein the serine at the seventh position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 109, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 110, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 114, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 117, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 119, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine and the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 123, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 126, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 128, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 129, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 131, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 132, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 135, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 140, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 141, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 143, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 144, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 146, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 147, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 148, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 149, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 150, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 154, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 155, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 157, wherein the serine at the fourth position of is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 158, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 159, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 160, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 161, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 162, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 164, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 165, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 167, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 170, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 172, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 173, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 174, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 176, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; and SEQ ID NO: 177, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine.
 43. A method of inducing a cellular immune response to a target peptide in a subject, the method comprising administering to the subject an effective amount of a target peptide selected from the group consisting of: SEQ ID NO: 3, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 5, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 6, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 7, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 12, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 13, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 14, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 15, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 20, wherein the threonine at the fifth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 22, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 24, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 26, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 28, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 29, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 30, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 31, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 32, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 33, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 34, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 43, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 48, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 49, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 50, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 52, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 54, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 56, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 61, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 63, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 67, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 68, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 71, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 72, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 73, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 81, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 85, wherein the serine at the seventh position of is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 87, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 91, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 93, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 94, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 95, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine, the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine, or any combination thereof; SEQ ID NO: 96, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 99, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 100, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 101, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 103, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 104, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 105, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 106, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 108, wherein the serine at the seventh position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 109, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 110, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 114, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 117, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 119, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine and the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 123, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 126, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 128, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 129, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 131, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 132, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 135, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 140, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 141, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 143, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 144, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 146, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 147, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 148, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 149, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 150, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 154, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 155, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 157, wherein the serine at the fourth position of is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 158, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 159, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 160, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 161, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 162, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 164, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 165, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 167, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 170, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 172, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 173, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 174, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 176, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; and SEQ ID NO: 177, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine.
 44. A kit comprising a first container containing at least one target peptide selected from group consisting of: SEQ ID NO: 3, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 5, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 6, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 7, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 12, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 13, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 14, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 15, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 20, wherein the threonine at the fifth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 22, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 24, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 26, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 28, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 29, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 30, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 31, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 32, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 33, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 34, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 43, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 48, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 49, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 50, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 52, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 54, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 56, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 61, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 63, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 67, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 68, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 71, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 72, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 73, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 81, wherein the serine at the ninth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 85, wherein the serine at the seventh position of is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 87, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 91, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 93, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 94, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 95, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine, the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine, or any combination thereof; SEQ ID NO: 96, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 99, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 100, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 101, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 103, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 104, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 105, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 106, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 108, wherein the serine at the seventh position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 109, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 110, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 114, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 117, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 119, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine and the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 123, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 126, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 128, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 129, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 131, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 132, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 135, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 140, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 141, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 143, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 144, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 146, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 147, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 148, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 149, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 150, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 154, wherein the serine at the third position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 155, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 157, wherein the serine at the fourth position of is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 158, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 159, wherein the serine at the eighth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 160, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 161, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 162, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 164, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 165, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 167, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 170, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 172, wherein the threonine at the fourth position is phosphorylated or replaced with a mimetic of phosphothreonine; SEQ ID NO: 173, wherein the serine at the fifth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 174, wherein the serine at the sixth position is phosphorylated or replaced with a mimetic of phosphoserine; SEQ ID NO: 176, wherein the serine at the fourth position is phosphorylated or replaced with a mimetic of phosphoserine; and SEQ ID NO: 177, wherein the threonine at the sixth position is phosphorylated or replaced with a mimetic of phosphothreonine; and a second container containing an adjuvant. 